General Information

    • DRAMP ID

    • DRAMP01920

    • Peptide Name

    • Brevinin-1CSa (Frogs, amphibians, animals)

    • Source

    • Rana cascadae (Cascades frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPILAGLAAKIVPKLFCLATKKC

    • Sequence Length

    • 24

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.17451843]Gram-negative bacterium: Escherichia coli (MIC=32 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=2 µM).

    • Hemolytic Activity

      • [Ref.17451843] LD50=5 µM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01920 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C123H208N28O26S2

    • Absent Amino Acids

    • DEHMNQRSWY

    • Common Amino Acids

    • L

    • Mass

    • 2559.3

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +4

    • Boman Index

    • 30.41

    • Hydrophobicity

    • 1.254

    • Aliphatic Index

    • 142.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 4

DRAMP01920

DRAMP01920 chydropathy plot

Comments Information

    • Brevinin-1CSa was strongly hemolytic against human erythrocytes.

Literature Information

  • ·Literature 1

    • Title

    • Peptide defenses of the Cascades frog Rana cascadae: implications for the evolutionary history of frogs of the Amerana species group.

    • Reference

    • Peptides. 2007 Jun;28(6):1268-1274.

    • Author

    • Conlon JM, Al-Dhaheri A, Al-Mutawa E, Al-Kharrge R, Ahmed E, Kolodziejek J, Nowotny N, Nielsen PF, Davidson C.