• DRAMP ID

    • DRAMP01941
    • Peptide Name

    • Brevinin-1CHb (Frogs, amphibians, animals)
    • Source

    • Rana chiricahuensis
    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)
    • Gene

    • Not found
    • Sequence

    • FLPVIAGLAAKVLPKLFCAITKKC
    • Sequence Length

    • 24
    • Protein Existence

    • Homology
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • Gram-negative bacteria: E. coli (MIC=10 µM), K. pneumoniae (MIC=40 µM), P. aeruginosa (MIC=10 µM);
      • Gram-positive bacterium: S. aureus (MIC=2.5 µM).
      • Yeast: C. albicans (MIC=2.5 µM).(Ref.2)
    • Hemolytic Activity

      • [Ref.21262304] LC50=5 µM against human erythrocytes
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)
    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP01941 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP01941.
    • Formula

    • C122H206N28O26S2
    • Absent Amino Acids

    • DEHMNQRSWY
    • Common Amino Acids

    • AKL
    • Mass

    • 2545.27
    • PI

    • 9.7
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 14
    • Net Charge

    • +4
    • Boman Index

    • 29.53
    • Hydrophobicity

    • 1.271
    • Aliphatic Index

    • 138.33
    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 125
    • Absorbance 280nm

    • 5.43
    • Polar Residues

    • 4

DRAMP01941

DRAMP01941 chydropathy plot
    • PTM

    • Contains disulfide bond.
  • ·Literature 1
    • Title

    • Selection for antimicrobial peptide diversity in frogs leads to gene duplication and low allelic variation.
    • Reference

    • J Mol Evol. 2007 Nov;65(5):605-615.
    • Author

    • Tennessen JA, Blouin MS.
  • ·Literature 2
    • Title

    • Characterization of antimicrobial peptides in skin secretions from discrete populations of Lithobates chiricahuensis (Ranidae) from central and southern Arizona.
    • Reference

    • Peptides. 2011 Apr;32(4):664-669.
    • Author

    • Conlon JM, Mechkarska M, Coquet L, Jouenne T, Leprince J, Vaudry H, Kolodziejek J, Nowotny N, King JD.