General Information

    • DRAMP ID

    • DRAMP01978

    • Peptide Name

    • Brevinin-2R (Frogs, amphibians, animals)

    • Source

    • Pelophylax ridibundus (Marsh frog) (Rana ridibunda)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • KLKNFAIGVAQSLLNKASCKLSGQC

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-cancer

    • Target Organism

    • [Swiss_Prot Entry P85095]Cytotoxic to cancer cells, acts via the activation of the lysosomal-mitochondrial death pathway and autophagy-like cell death

    • Hemolytic Activity

      • [Ref.18494941]2.5% hemolytic activity at 200 μg/ml against sheep erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01978 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01978.

Physicochemical Information

    • Formula

    • C114H197N33O33S2

    • Absent Amino Acids

    • DEHMPRTWY

    • Common Amino Acids

    • KL

    • Mass

    • 2622.14

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +4

    • Boman Index

    • -15.27

    • Hydrophobicity

    • 0.172

    • Aliphatic Index

    • 101.6

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.21

    • Polar Residues

    • 9

DRAMP01978

DRAMP01978 chydropathy plot

Comments Information

    • Function

    • Cytotoxicity towards malignant cells, including Jurkat (T-cell leukemia), BJAB (B-cell lymphoma), HT29/219, SW742 (colon carcinomas), L929 (fibrosarcoma), MCF-7 (breast adenocarcinoma), A549 (lung carcinoma), acts via the activation of the lysosomal-mitochondrial death pathway and autophagy-like cell death. Does not show significant hemolytic activity. Tissue specificity

Literature Information

  • ·Literature 1

    • Title

    • Brevinin-2R(1) semi-selectively kills cancer cells by a distinct mechanism, which involves the lysosomal-mitochondrial death pathway.

    • Reference

    • Cell Mol Med. 2008 Jun;12(3):1005-1022.

    • Author

    • Ghavami S, Asoodeh A, Klonisch T, Halayko AJ, Kadkhoda K, Kroczak TJ, Gibson SB, Booy EP, Naderi-Manesh H, Los M.