General Information

    • DRAMP ID

    • DRAMP02001

    • Peptide Name

    • Brevinin-1HN1 (Frogs, amphibians, animals)

    • Source

    • Odorrana hainanensis (Hainan odorous frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPLIASLAANFVPKIFCKITKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.22450466]Gram-positive bacteria: Enterococcus faecium 091299 (MIC=19 µM), Enterococcus faecalis 981 (MIC=19 µM), Staphylococcus aureus ATCC2592 (MIC=1.2 µM), Staphylococcus carnosus KHS (MIC=4.8 µM), Bacillus licheniformis X39 (MIC=2.4 µM), Rhodococcus rhodochrous X15 (MIC=1.2 µM);
      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=150 µM), Psychrobacter faecalis X29 (MIC=4.8 µM);
      • Fungi: Slime mould 090223 (MIC=1.2 µM), Candida albicans ATCC2002 (MIC=2.4 µM).

    • Hemolytic Activity

      • [Ref.22450466] LC50=75 µM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02001 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02001.

Physicochemical Information

    • Formula

    • C128H209N29O28S2

    • Absent Amino Acids

    • DEGHMQRWY

    • Common Amino Acids

    • K

    • Mass

    • 2666.36

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • 15.68

    • Hydrophobicity

    • 1.004

    • Aliphatic Index

    • 122.08

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 5

DRAMP02001

DRAMP02001 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity against Gram-positive bacteria and fungi but has weak or no activity against a range of Gram-negative bacteria except P. faecalis. Has hemolytic activity against human erythrocytes (LC50=75 µM).

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • Contains one disulfide bond.

Literature Information

  • ·Literature 1

    • Title

    • Novel antimicrobial peptides isolated from the skin secretions of Hainan odorous frog, Odorrana hainanensis.

    • Reference

    • Peptides. 2012 Jun;35(2):285-290.

    • Author

    • Wang H, Yu Z, Hu Y, Li F, Liu L, Zheng H, Meng H, Yang S, Yang X, Liu J.