• • DRAMP ID

  • DRAMP02004

• • Peptide Name

  • Brevinin-1V (Frogs, amphibians, animals)

• • Source

  • Odorrana versabilis (Chinese bamboo leaf odorous frog) (Rana versabilis)

• • Family

  • Belongs to the frog skin active peptide family (Brevinin subfamily)

• • Gene

  • Not found

• • Sequence

  • FLPLIASVAANLVPKIFCKITKKC

• • Sequence Length

  • 24

• • UniProt Entry

  • Q2UXV8,E7EKC5

• • Protein Existence

  • Transcript level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.22450466]Gram-positive bacteria: Staphylococcus aureus ATCC2592 (MIC=19 µM), Bacillus licheniformis X39 (MIC=37.5 µM), Rhodococcus rhodochrous X15 (MIC=9.5 µM), Enterococcus faecium 091299 (MIC=19 µM), E. faecalis 981 (MIC=19 µM);
    • Gram-negative bacterium: Psychrobacter faecalis X29 (MIC=9.5 µM);
    • Fungi: Slime mould 090223 (MIC=1.2 µM), Candida albicans ATCC2002 (MIC=19-37.5 µM).

• • Hemolytic Activity

  • • [Ref.22450466] LC50=75 µM against human erythrocytes

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Cyclization (Cys18 and Cys24)

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys18 and Cys24.

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP02004.

DRAMP02004

• • Function

  • Has antimicrobial activity against Gram-positive bacteria and fungi but has weak or no activity against a range of Gram-negative bacteria except P. faecalis. Has hemolytic activity against human erythrocytes.

• • Tissue specificity

  • Expressed by the skin glands.

• • PTM

  • Contains one disulfide bond.

• ·Literature 1

• • Title

  • Amphibian skin peptides and their corresponding cDNAs from single lyophilized secretion samples: identification of novel brevinins from three species of Chinese frogs.

• • Pubmed ID

  • 16139929

• • Reference

  • Peptides. 2006 Jan;27(1):42-48.

• • Author

  • Chen T, Li L, Zhou M, Rao P, Walker B, Shaw C.

• ·Literature 2

• • Title

  • Novel antimicrobial peptides isolated from the skin secretions of Hainan odorous frog, Odorrana hainanensis.

• • Pubmed ID

  • 22450466

• • Reference

  • Peptides. 2012 Jun;35(2):285-290.

• • Author

  • Wang H, Yu Z, Hu Y, Li F, Liu L, Zheng H, Meng H, Yang S, Yang X, Liu J.