General Information

    • DRAMP ID

    • DRAMP02023

    • Peptide Name

    • Brevinin-2DYd (Frogs, amphibians, animals)

    • Source

    • Rana dybowskii (Dybovsky's frog) (Korean brown frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GIFDVVKGVLKGVGKNVAGSLLEQLKCKLSGGC

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus (MIC=15 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=15 µM).
      • NOTE: mixture of Brevinin-2DYc/2DYd

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys27 and Cys33)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys27 and Cys33.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02023 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C147H254N40O42S2

    • Absent Amino Acids

    • HMPRTWY

    • Common Amino Acids

    • G

    • Mass

    • 3318

    • PI

    • 9.24

    • Basic Residues

    • 5

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • 1.39

    • Hydrophobicity

    • 0.491

    • Aliphatic Index

    • 117.88

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.91

    • Polar Residues

    • 12

DRAMP02023

DRAMP02023 chydropathy plot

Comments Information

    • Function

    • Antimicrobial peptide.

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • contains one disulfide bond 27-33.

Literature Information

  • ·Literature 1

    • Title

    • Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii.

    • Reference

    • Toxicon. 2007 Nov;50(6):746-756.

    • Author

    • Conlon JM, Kolodziejek J, Nowotny N, Leprince J, Vaudry H, Coquet L, Jouenne T, Iwamuro S.