General Information

    • DRAMP ID

    • DRAMP02026

    • Peptide Name

    • Brevinin-1CDYa (Frogs, amphibians, animals)

    • Source

    • Rana dybowskii (Dybovsky's frog) (Korean brown frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • LLSLALAALPKLFCLIFKKC

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Swiss_Prot Entry B3VZU0]Gram-positive bacterium: Staphylococcus aureus (MIC=12.5 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=25 µM)

    • Hemolytic Activity

      • [Ref.19539775]HC50=125 μM against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys14 and Cys20)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys14 and Cys20.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02026 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C107H181N23O22S2

    • Absent Amino Acids

    • DEGHMNQRTVWY

    • Common Amino Acids

    • L

    • Mass

    • 2205.87

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • 33.26

    • Hydrophobicity

    • 1.65

    • Aliphatic Index

    • 171

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.58

    • Polar Residues

    • 3

DRAMP02026

DRAMP02026 chydropathy plot

Comments Information

    • Function

    • Has low activity against the Gram-positive bacteria

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • contains one disulfide bond 14-20.

Literature Information

  • ·Literature 1

    • Title

    • Characterization of antimicrobial peptides isolated from the skin of the Chinese frog, Rana dybowskii.

    • Reference

    • Comp Biochem Physiol B Biochem Mol Biol. 2009 Oct;154(2):174-178.

    • Author

    • Jin LL, Li Q, Song SS, Feng K, Zhang DB, Wang QY, Chen YH.