General Information

    • DRAMP ID

    • DRAMP02032

    • Peptide Name

    • Brevinin-1TSa (Frogs, amphibians, animals)

    • Source

    • Rana tsushimensis (Tsushima brown frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLGSIVGALASALPSLISKIRN

    • Sequence Length

    • 22

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.16413829]Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=25 µM), Klebsiella pneumoniae KK3 9904 (MIC>100 µM), Enterobacter cloacae HNTCC 53001 (MIC=50 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=50 µM), Proteus mirabilis ATCC 25933 (MIC>100 µM);
      • Gram-positive bacteria: Staphylococcus aureus NCTC 8325 (MIC=25 µM), Methicillin-resistant Staphylococcus aureus T7/20 (MIC=25 µM), Staphylococcus epidermidis RP62A (MIC=12.5 µM), Enterococcus faecalis ATCC 29212 (MIC=50 µM), Streptococcus group B HNTCC 80130 (MIC=50 µM);
      • Yeast: Candida albicans ATCC 90028 (MIC=50 µM)

    • Hemolytic Activity

      • [Ref.16413829]LD50=12 μM against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02032 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C102H175N27O28

    • Absent Amino Acids

    • CDEHMQTWY

    • Common Amino Acids

    • LS

    • Mass

    • 2227.68

    • PI

    • 11

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +2

    • Boman Index

    • 8.06

    • Hydrophobicity

    • 1.073

    • Aliphatic Index

    • 150.91

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 7

DRAMP02032

DRAMP02032 chydropathy plot

Comments Information

    • Function

    • Activity against a range of Gram-positive and Gram-negative bacteria, but hemolytic activity against human erythrocytes.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from the skin of the Tsushima brown frog Rana tsushimensis.

    • Reference

    • Comp Biochem Physiol C Toxicol Pharmacol. 2006 May;143(1):42-49.

    • Author

    • Conlon JM, Al-Ghaferi N, Abraham B, Sonnevend A, Coquet L, Leprince J, Jouenne T, Vaudry H, Iwamuro S.