General Information

    • DRAMP ID

    • DRAMP02034

    • Peptide Name

    • Brevinin-1AUa (Frogs, amphibians, animals)

    • Source

    • Rana aurora aurora (Northern red-legged frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPILAGLAAKLVPKVFCSITKKC

    • Sequence Length

    • 24

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.15325526]Gram-negative bacteria: Escherichia coli (MIC=13 µM), Pseudomonas aeruginosa (MIC=25 µM), Enterobacter cloacae (MIC=13 µM), Klebsiella pneumoniae (MIC=50 µM), Proteus mirabilis (MIC>50 µM);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=3 µM), Staphylococcus epidermidis (MIC=6 µM), Enterococcus faecalis (MIC=13 µM), Streptococcus Group B (MIC=13 µM);
      • Yeast: Candida albicans (MIC>40 µM).

    • Hemolytic Activity

      • [Ref.15325526] HC50=5 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02034 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C122H206N28O27S2

    • Absent Amino Acids

    • DEHMNQRWY

    • Common Amino Acids

    • KL

    • Mass

    • 2561.27

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • 24.32

    • Hydrophobicity

    • 1.163

    • Aliphatic Index

    • 134.17

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 5

DRAMP02034

DRAMP02034 chydropathy plot

Comments Information

    • Function

    • Activity against a range of Gram-positive and Gram-negative bacteria, and also shows hemolytic.

Literature Information

  • ·Literature 1

    • Title

    • Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora.

    • Reference

    • Dev Comp Immunol. 2005;29(1):83-90.

    • Author

    • Conlon JM, Sonnevend A, Davidson C, Demandt A, Jouenne T.