General Information
-
DRAMP ID
- DRAMP02035
-
Peptide Name
- Brevinin-1AUb (Frogs, amphibians, animals)
-
Source
- Rana aurora aurora (Northern red-legged frog)
-
Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
-
Gene
- Not found
-
Sequence
- FLPILAGLAANILPKVFCSITKKC
-
Sequence Length
- 24
-
UniProt Entry
- No entry found
-
Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
-
Target Organism
-
- [Ref.15325526]Gram-negative bacteria: Escherichia coli (MIC=25 µM), Pseudomonas aeruginosa (MIC=25 µM), Enterobacter cloacae (MIC=25 µM), Klebsiella pneumoniae (MIC>50 µM), Proteus mirabilis (MIC>50 µM);
- Gram-positive bacteria: Staphylococcus aureus (MIC=3 µM), Staphylococcus epidermidis (MIC=6 µM), Enterococcus faecalis (MIC=6 µM), Streptococcus Group B (MIC=13 µM);
- Yeast: Candida albicans (MIC=3 µM).
-
Hemolytic Activity
-
- [Ref.15325526] HC50=7 μM against human erythrocytes
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Cyclization (Cys18 and Cys24)
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys18 and Cys24.
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- Please click DRAMP02035_predicted_structure.pdb to download.
Physicochemical Information
-
Formula
- C121H202N28O28S2
Absent Amino Acids
- DEHMQRWY
Common Amino Acids
- L
Mass
- 2561.23
PI
- 9.39
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 13
Net Charge
- +3
-
Boman Index
- 24.11
Hydrophobicity
- 1.192
Aliphatic Index
- 138.33
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 5.43
Polar Residues
- 6
DRAMP02035
Comments Information
Function
- Activity against a range of Gram-positive and Gram-negative bacteria, and also shows hemolytic activity.
Literature Information
- ·Literature 1
-
Title
- Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora.
-
Pubmed ID
- 15325526
-
Reference
- Dev Comp Immunol. 2005;29(1):83-90.
-
Author
- Conlon JM, Sonnevend A, Davidson C, Demandt A, Jouenne T.