General Information

    • DRAMP ID

    • DRAMP02037

    • Peptide Name

    • Brevinin-2-RN2 (Frogs, amphibians, animals)

    • Source

    • Rana nigrovittata

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GAFGNFLKGVAKKAGLKILSIAQCKLFGTC

    • Sequence Length

    • 30

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus ATCC2592 (MIC=6.25 µg/ml), Staphylococcus aureus ATCC43300 (MIC=6.25 µg/ml), Bacillus subtilis (MIC=12.5 µg/ml);
      • Gram-negative bacteria: Escherichia coli ML-35P (MIC=50 µg/ml), Pseudomonas aeruginosa PA01 (MIC=12.5 µg/ml), Pseudomonas aeruginosa ATCC27853 (MIC=12.5 µg/ml). Yeast: Candida albicans ATCC2002 (MIC=3.1 µg/m).

    • Hemolytic Activity

      • [Ref.21171145] It has 21% hemolysis at 100 µg/ml against rabbit red blood cell

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys24 and Cys30)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys24 and Cys30.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02037 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C142H235N37O35S2

    • Absent Amino Acids

    • DEHMPRWY

    • Common Amino Acids

    • GK

    • Mass

    • 3084.77

    • PI

    • 9.9

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +5

    • Boman Index

    • 11.1

    • Hydrophobicity

    • 0.633

    • Aliphatic Index

    • 101

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.31

    • Polar Residues

    • 10

DRAMP02037

DRAMP02037 chydropathy plot

Comments Information

    • Brevinin-2-RN1 and -RN2 could induce 17 and 21% Rabbit red blood cell hemolysis, respectively.

Literature Information

  • ·Literature 1

    • Title

    • Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata.

    • Reference

    • J Pept Sci. 2011 Jan;17(1):68-72.

    • Author

    • Liu X, Liu R, Wei L, Yang H, Zhang K, Liu J, Lai R.