General Information

    • DRAMP ID

    • DRAMP02044

    • Peptide Name

    • Brevinin-1-OR5 (Frogs, amphibians, animals)

    • Source

    • Odorrana rotodora (Chinese odorous frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • ILPFVAGVAAEMMKHVYCAASKKC

    • Sequence Length

    • 24

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=15.2 µg/ml), Bacillus pyocyaneus CMCCB 10104 (MIC=30.4 µg/ml);
      • Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=15.2 µg/ml).
      • Yeast: Candida albicans (MIC=7.6 µg/ml).

    • Hemolytic Activity

      • [Ref.22029824] It shows no hemolytic activity against human red blood cells at the concentration of 50 μg/mL.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02044 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C115H187N29O29S4

    • Absent Amino Acids

    • DNQRTW

    • Common Amino Acids

    • A

    • Mass

    • 2568.17

    • PI

    • 8.82

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +3

    • Boman Index

    • 10.53

    • Hydrophobicity

    • 0.792

    • Aliphatic Index

    • 89.58

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1615

    • Absorbance 280nm

    • 70.22

    • Polar Residues

    • 5

DRAMP02044

DRAMP02044 chydropathy plot

Comments Information

    • It differs from AP1824 only at position 14

    • Q vs. K.

Literature Information

  • ·Literature 1

    • Title

    • Extremely abundant antimicrobial peptides existed in the skins of nine kinds of Chinese odorous frogs.

    • Reference

    • J Proteome Res. 2012 Jan 1;11(1):306-319.

    • Author

    • Yang X, Lee WH, Zhang Y.