• DRAMP ID

    • DRAMP02084
    • Peptide Name

    • Brevinin-2E (Frogs, amphibians, animals)
    • Source

    • Rana esculenta (Edible frog) (Pelophylax esculentus)
    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)
    • Gene

    • Not found
    • Sequence

    • GIMDTLKNLAKTAGKGALQSLLNKASCKLSGQC
    • Sequence Length

    • 33
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • [Ref.8508915]Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=0.2 µM), Staphylococcus aureus Cowan 1 (MIC=2.0 µM);
      • Gram-negative bacteria: Escherichia coli D21 (MIC=0.5 µM), Pseudomonas aeruginosa ATCC 15692 (MIC>30 µM).
    • Hemolytic Activity

      • [Ref.8163497]LC50>100 μM against human red blood cells
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Cyclization (Cys27 and Cys33)
    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys27 and Cys33.
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP02084 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • Formula

    • C142H252N42O45S3
    • Absent Amino Acids

    • EFHPRVWY
    • Common Amino Acids

    • L
    • Mass

    • 3364
    • PI

    • 9.6
    • Basic Residues

    • 5
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 11
    • Net Charge

    • +4
    • Boman Index

    • -25.82
    • Hydrophobicity

    • -0.03
    • Aliphatic Index

    • 94.85
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 125
    • Absorbance 280nm

    • 3.91
    • Polar Residues

    • 13

DRAMP02084

DRAMP02084 chydropathy plot
    • Function

    • Shows antibacterial activity against representative Gram-negative and Gram-positive bacterial species, and hemolytic activity.
  • ·Literature 1
    • Title

    • Novel antimicrobial peptides from skin secretion of the European frog Rana esculenta.
    • Reference

    • FEBS Lett. 1993 Jun 14;324(2):159-161.
    • Author

    • Simmaco M, Mignogna G, Barra D, Bossa F.