General Information
-
DRAMP ID
- DRAMP02084
-
Peptide Name
- Brevinin-2E (Frogs, amphibians, animals)
-
Source
- Rana esculenta (Edible frog) (Pelophylax esculentus)
-
Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
-
Gene
- Not found
-
Sequence
- GIMDTLKNLAKTAGKGALQSLLNKASCKLSGQC
-
Sequence Length
- 33
-
UniProt Entry
- P32413
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.8508915]Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=0.2 µM), Staphylococcus aureus Cowan 1 (MIC=2.0 µM);
- Gram-negative bacteria: Escherichia coli D21 (MIC=0.5 µM), Pseudomonas aeruginosa ATCC 15692 (MIC>30 µM).
-
Hemolytic Activity
-
- [Ref.8163497]LC50>100 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Cyclization (Cys27 and Cys33)
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys27 and Cys33.
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- Please click DRAMP02084_predicted_structure.pdb to download.
Physicochemical Information
-
Formula
- C142H252N42O45S3
Absent Amino Acids
- EFHPRVWY
Common Amino Acids
- L
Mass
- 3364
PI
- 9.6
Basic Residues
- 5
Acidic Residues
- 1
Hydrophobic Residues
- 11
Net Charge
- +4
-
Boman Index
- -25.82
Hydrophobicity
- -0.03
Aliphatic Index
- 94.85
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 3.91
Polar Residues
- 13
DRAMP02084
Comments Information
Function
- Shows antibacterial activity against representative Gram-negative and Gram-positive bacterial species, and hemolytic activity.
Literature Information
- ·Literature 1
-
Title
- Novel antimicrobial peptides from skin secretion of the European frog Rana esculenta.
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Pubmed ID
- 8508915
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Reference
- FEBS Lett. 1993 Jun 14;324(2):159-161.
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Author
- Simmaco M, Mignogna G, Barra D, Bossa F.