General Information

    • DRAMP ID

    • DRAMP02086

    • Peptide Name

    • Brevinin-1PRa (frogs, amphibians, animals)

    • Source

    • Rana pretiosa (North oregon spotted frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPVLTGLTPSIVPKLVCLLTKKC

    • Sequence Length

    • 24

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.15003829] Gram-negative bacterium: Escherichia coli(MIC>100μM);
      • Gram-positive bacterium: Staphylococcus aureus(MIC=13μM);
      • Yeast: Candida albicans(MIC=100μM).[Ref.21295070] Gram-negative bacterium: Escherichia coli(MIC>100μM);
      • Gram-positive bacterium: Staphylococcus aureus(MIC=50μM);
      • Yeast: Candida albicans(MIC=100μM).

    • Hemolytic Activity

      • [Ref.15003829] HC50=7μM against human erythrocytes.
      • [Ref.21295070] LC50= 150μM against human erythrocytes.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02086 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02086.

Physicochemical Information

    • Formula

    • C122H211N27O29S2

    • Absent Amino Acids

    • ADEHMNQRWY

    • Common Amino Acids

    • L

    • Mass

    • 2584.3

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +3

    • Boman Index

    • 25.28

    • Hydrophobicity

    • 1.163

    • Aliphatic Index

    • 150

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 7

DRAMP02086

DRAMP02086 chydropathy plot

Comments Information

    • Function

    • activity against reference strains of Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella pneumoniae) and Gram-positive (Staphlococcus aureus) bacteria but displayed relatively low hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica.

    • Reference

    • Regul Pept. 2004 May 15;118(3):135-141.

    • Author

    • Conlon JM, Sonnevend A, Patel M, Al-Dhaheri K, Nielsen PF, Kolodziejek J, Nowotny N, Iwamuro S, P
  • ·Literature 2

    • Title

    • Host defense peptides in skin secretions of the Oregon spotted frog Rana pretiosa: implications for species resistance to chytridiomycosis.

    • Reference

    • Dev Comp Immunol. 2011 Jun;35(6):644-649.

    • Author