General Information
-
DRAMP ID
- DRAMP02104
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Peptide Name
- Brevinin-2RTa (Frogs, amphibians, animals)
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Source
- Amolops ricketti (Chinese sucker frog)
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Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
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Gene
- Not found
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Sequence
- GLMSTLKDFGKTAAKEIAQSLLSTASCKLAKTC
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Sequence Length
- 33
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UniProt Entry
- No entry found
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Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.22100518]Gram-positive bacteria: Staphylococcus aureus ATCC2592 (MIC=12.5 µg/ml), Bacillus licheniformis X39 (MIC=50.0 µg/ml), Staphylococcus carnosus KHS (MIC=6.3 µg/ml), Rhodococcus rhodochrous X15 (MIC=1.6 µg/ml);
- Gram-negative bacteria: Serratia rubidaea X01 (MIC=100.0 µg/ml), Psychrobacter faecalis X29 (MIC=6.3 µg/ml).
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Hemolytic Activity
-
- [Ref.22100518]LD50>400 μg/ml against rabbit red blood cells
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP02104.
Physicochemical Information
-
Formula
- C147H255N39O47S3
Absent Amino Acids
- HNPRVWY
Common Amino Acids
- AKL
Mass
- 3417.05
PI
- 9.24
Basic Residues
- 5
Acidic Residues
- 2
Hydrophobic Residues
- 12
Net Charge
- +3
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Boman Index
- -24.36
Hydrophobicity
- 0.164
Aliphatic Index
- 86.06
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 3.91
Polar Residues
- 12
DRAMP02104
Comments Information
Function
- Brevinin-2RTb has antibacterial activity and hemolytic activity against rabbit red blood cells in vitro.
PTM
- Contains one disulfide bond 27-33.
Literature Information
- ·Literature 1
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Title
- Identification and characterization of antimicrobial peptides from skin of Amolops ricketti (Anura: Ranidae).
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Pubmed ID
- 22100518
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Reference
- Peptides. 2012 Jan;33(1):27-34.
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Author
- Wang H, Ran R, Yu H, Yu Z, Hu Y, Zheng H, Wang D, Yang F, Liu R, Liu J.