General Information

    • DRAMP ID

    • DRAMP02105

    • Peptide Name

    • Brevinin-2RTb (Frogs, amphibians, animals)

    • Source

    • Amolops ricketti (Chinese sucker frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GILDTLKEFGKTAAKGIAQSLLSTASCKLAKTC

    • Sequence Length

    • 33

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.22100518]Gram-positive bacteria: Staphylococcus aureus ATCC2592 (MIC=50.0 µg/ml), Bacillus licheniformis X39 (MIC=200.0 µg/ml), Staphylococcus carnosus KHS (MIC=100.0 µg/ml), Rhodococcus rhodochrous X15 (MIC=12.5 µg/ml);
      • Gram-negative bacteria: Psychrobacter faecalis X29 (MIC=12.5 µg/ml).

    • Hemolytic Activity

      • [Ref.22100518]LD50>400 μg/ml against rabbit red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02105 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02105.

Physicochemical Information

    • Formula

    • C147H255N39O46S2

    • Absent Amino Acids

    • HMNPRVWY

    • Common Amino Acids

    • AKL

    • Mass

    • 3369

    • PI

    • 9.24

    • Basic Residues

    • 5

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • -17.45

    • Hydrophobicity

    • 0.255

    • Aliphatic Index

    • 97.88

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.91

    • Polar Residues

    • 12

DRAMP02105

DRAMP02105 chydropathy plot

Comments Information

    • Function

    • Brevinin-2RTb has antibacterial activity and relatively low hemolytic activity (>400 µg/ml) against rabbit red blood cells in vitro.

    • PTM

    • Contains one disulfide bond 27-33.

Literature Information

  • ·Literature 1

    • Title

    • Identification and characterization of antimicrobial peptides from skin of Amolops ricketti (Anura: Ranidae).

    • Reference

    • Peptides. 2012 Jan;33(1):27-34.

    • Author

    • Wang H, Ran R, Yu H, Yu Z, Hu Y, Zheng H, Wang D, Yang F, Liu R, Liu J.