General Information

    • DRAMP ID

    • DRAMP02125

    • Peptide Name

    • Hylin-a1 (Hy-a1; Frogs, amphibians, animals)

    • Source

    • Hypsiboas albopunctatus (Spotted tree frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IFGAILPLALGALKNLIK

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.19056441]Gram-positive bacteria: Staphylococcus aureus ATCC 25926 (MIC=8 µM), Enterococcus faecalis ATCC 29212 (MIC=16 µM), Bacillus subtilis ATCC 19659 (MIC=8 µM);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=32 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=64 µM);
      • Fungi: Candida albicans ATCC 90028 (MIC=16.7 µM), C. krusei ATCC 6258 (MIC=16.7 µM), C. parapsilosis ATCC 22019 (MIC=67 µM), Cryptococcus neoformans ATCC 90012 (MIC=33.5 µM)

    • Hemolytic Activity

      • [Ref.19056441]HC50=18 μM against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02125 helical wheel diagram

    • PDB ID

    • 2N0O resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP02125.

Physicochemical Information

    • Formula

    • C91H157N21O20

    • Absent Amino Acids

    • CDEHMQRSTVWY

    • Common Amino Acids

    • L

    • Mass

    • 1865.38

    • PI

    • 10

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +2

    • Boman Index

    • 31.91

    • Hydrophobicity

    • 1.5

    • Aliphatic Index

    • 190

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP02125

DRAMP02125 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity against the Gram-positive bacteria and the Gram-negative bacteria. Has hemolytic activity against human erythrocytes.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Hylin a1, the first cytolytic peptide isolated from the arboreal South American frog Hypsiboas albopunctatus (spotted treefrog).

    • Reference

    • Peptides. 2009 Feb;30(2):291-296.

    • Author

    • Castro MS, Ferreira TC, Cilli EM, Crusca E Jr, Mendes-Giannini MJ, Sebben A, Ricart CA, Sousa MV, Fontes W.