General Information

    • DRAMP ID

    • DRAMP02127

    • Peptide Name

    • Leptoglycin (Gly-rich; Frogs, amphibians, animals)

    • Source

    • Leptodactylus pentadactylus (Smokey jungle frog) (Rana pentadactyla)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLLGGLLGPLLGGGGGGGGGLL

    • Sequence Length

    • 22

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.19298834]Gram-negative bacteria: Escherichia coli ATCC 28922 (MIC=50 µM), Pseudomonas aeruginosa ATCC 9027 (MIC=8 µM);
      • Fungi: Citrobacter freundii ATCC 8090 (MIC=75 µM)

    • Hemolytic Activity

      • [Ref.19298834]Non-hemolytic activity was observed at 2-200 μM concentration

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02127 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02127.

Physicochemical Information

    • Formula

    • C79H136N22O23

    • Absent Amino Acids

    • ACDEFHIKMNQRSTVWY

    • Common Amino Acids

    • G

    • Mass

    • 1762.08

    • PI

    • 5.52

    • Basic Residues

    • 0

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • 0

    • Boman Index

    • 51.58

    • Hydrophobicity

    • 1.073

    • Aliphatic Index

    • 141.82

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 13

DRAMP02127

DRAMP02127 chydropathy plot

Comments Information

    • Function

    • Antimicrobial protein. Has antibacterial activity but not against Gram-positive bacteria Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis, yeasts Candida albicans and Candida tropicalis and dermatophytes fungi Microsporum canis and Trichophyton rubrum. No hemolytic activity was observed at the 2-200 microM range concentration.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Leptoglycin: a new glycine/leucine-rich antimicrobial peptide isolated from the skin secretion of the South American frog Leptodactylus pentadactylus (Leptodactylidae).

    • Reference

    • Toxicon. 2009 Jul;54(1):23-32.

    • Author

    • Sousa JC, Berto RF, Gois EA, Fontenele-Cardi NC, Honório JE Jr, Konno K, Richardson M, Rocha MF, Camargo AA, Pimenta DC, Cardi BA, Carvalho KM.