General Information

    • DRAMP ID

    • DRAMP02133

    • Peptide Name

    • Antimicrobial peptide 4 (XT-4; Frogs, amphibians, animals)

    • Source

    • Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GVFLDALKKFAKGGMNAVLNPK

    • Sequence Length

    • 22

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.11738090]Gram-positive bacteria: Staphylococcus aureus (MIC>100 µM);
      • Gram-negative bacteria: Escherichia coli (MIC=18 µM);
      • Fungi: Candida albicans (MIC>100 µM).

    • Hemolytic Activity

      • [Ref.11738090]HC50>150 μM against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02133 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C107H176N28O27S

    • Absent Amino Acids

    • CEHIQRSTWY

    • Common Amino Acids

    • K

    • Mass

    • 2318.81

    • PI

    • 10

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • -4.8

    • Hydrophobicity

    • 0.173

    • Aliphatic Index

    • 93.18

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP02133

DRAMP02133 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity against Gram-positive bacterium S. aureus and yeast C. albicans, and very weak activity against Gram-negative bacterium E.coli. Has strong hemolytic activity against human red blood cells.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides isolated from skin secretions of the diploid frog, Xenopus tropicalis (Pipidae).

    • Reference

    • Biochim Biophys Acta. 2001 Nov 26;1550(1):81-89.

    • Author

    • Ali MF, Soto A, Knoop FC, Conlon JM.