General Information
-
DRAMP ID
- DRAMP02230
-
Peptide Name
- Ranatuerin-3 (Frogs, amphibians, animals)
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Source
- Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)
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Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
-
Gene
- Not found
-
Sequence
- GFLDIINKLGKTFAGHMLDKIKCTIGTCPPSP
-
Sequence Length
- 32
-
UniProt Entry
- P82780
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.9784389] Gram-positive bacterium: Staphylococcus aureus (MIC=60 µM).
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Hemolytic Activity
-
- [Ref.9784389] Have no detectable hemolytic activity at aconcentration of 20µg/ml against human red blood cell.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bridge between Cys23 and Cys28.
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Stereochemistry
- L
-
Structure
- Alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP02230.
Physicochemical Information
-
Formula
- C154H251N39O42S3
Absent Amino Acids
- EQRVWY
Common Amino Acids
- GIK
Mass
- 3417.1
PI
- 8.82
Basic Residues
- 5
Acidic Residues
- 2
Hydrophobic Residues
- 10
Net Charge
- +3
-
Boman Index
- -11.17
Hydrophobicity
- 0.159
Aliphatic Index
- 88.44
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 4.03
Polar Residues
- 11
DRAMP02230
Comments Information
Function
- Antibacterial activity against Gram-positive bacterium S.aureus. Has no detectable hemolytic activity.
Literature Information
- ·Literature 1
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Title
- Ranatuerins: antimicrobial peptides isolated from the skin of the American bullfrog, Rana catesbeiana.
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Pubmed ID
- 9784389
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Reference
- Biochem Biophys Res Commun. 1998 Sep 29;250(3):589-592.
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Author
- Goraya J, Knoop FC, Conlon JM.