General Information
-
DRAMP ID
- DRAMP02259
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Peptide Name
- Magainin 2 analogue (F5Y, F16W-MG2)
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Source
- Xenopus laevis (African clawed frog)
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Family
- Belongs to the gastrin/cholecystokinin family (Magainin subfamily)
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Gene
- Not found
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Sequence
- GIGKYLHSAKKFGKAWVGEIMNS
-
Sequence Length
- 23
-
UniProt Entry
- P11006
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Unknown
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Target Organism
- No MICs found in DRAMP database
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Alpha helix (1 helices; 12 residues)
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Structure Description
- The peptide assumed α-helices in the lipid environment, as characterized by double minima around 208 and 222 nm. The [θ]222 value, a measure of helicity, of I (−17,900 deg cm2 dmol−1) was ca. 20% smaller than that of F16W-MG2 (−23,200 deg cm2 dmol−1).
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Helical Wheel Diagram
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PDB ID
- 1DUM resolved by NMR
- 1DUM-> 
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Predicted Structure
- There is no predicted structure for DRAMP02259.
Physicochemical Information
-
Formula
- C116H181N31O30S
Absent Amino Acids
- CDPQRT
Common Amino Acids
- GK
Mass
- 2521.96
PI
- 9.83
Basic Residues
- 5
Acidic Residues
- 1
Hydrophobic Residues
- 8
Net Charge
- +4
-
Boman Index
- -13.41
Hydrophobicity
- -0.257
Aliphatic Index
- 72.17
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 6990
Absorbance 280nm
- 317.73
Polar Residues
- 8
DRAMP02259
Comments Information
Membrane Permeabilization
- The peptide-induced membrane permeabilization was examined on the basis of the efflux of the fluorescent dye calcein from egg PG LUVs. The mutant I (F5Y, F16W-MG2) showed a slightly weaker but comparable leakage activity compared with F16W-MG2.
Literature Information
- ·Literature 1
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Title
- Effects of peptide dimerization on pore formation: Antiparallel disulfide-dimerized magainin 2 analogue.
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Pubmed ID
- 11180056
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Reference
- Biopolymers. 2001 Apr 5;58(4):437-446.
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Author
- Hara T, Kodama H, Kondo M, Wakamatsu K, Takeda A, Tachi T, Matsuzaki K.