General Information

    • DRAMP ID

    • DRAMP02259

    • Peptide Name

    • Magainin 2 analogue (F5Y, F16W-MG2)

    • Source

    • Xenopus laevis (African clawed frog)

    • Family

    • Belongs to the gastrin/cholecystokinin family (Magainin subfamily)

    • Gene

    • Not found

    • Sequence

    • GIGKYLHSAKKFGKAWVGEIMNS

    • Sequence Length

    • 23

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Unknown

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix (1 helices; 12 residues)

    • Structure Description

    • The peptide assumed α-helices in the lipid environment, as characterized by double minima around 208 and 222 nm. The [θ]222 value, a measure of helicity, of I (−17,900 deg cm2 dmol−1) was ca. 20% smaller than that of F16W-MG2 (−23,200 deg cm2 dmol−1).

    • Helical Wheel Diagram

    • DRAMP02259 helical wheel diagram

    • PDB ID

    • 1DUM resolved by NMR

    • Predicted Structure

    • There is no predicted structure for DRAMP02259.

Physicochemical Information

    • Formula

    • C116H181N31O30S

    • Absent Amino Acids

    • CDPQRT

    • Common Amino Acids

    • GK

    • Mass

    • 2521.96

    • PI

    • 9.83

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • -13.41

    • Hydrophobicity

    • -0.257

    • Aliphatic Index

    • 72.17

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 317.73

    • Polar Residues

    • 8

DRAMP02259

DRAMP02259 chydropathy plot

Comments Information

    • Membrane Permeabilization

    • The peptide-induced membrane permeabilization was examined on the basis of the efflux of the fluorescent dye calcein from egg PG LUVs. The mutant I (F5Y, F16W-MG2) showed a slightly weaker but comparable leakage activity compared with F16W-MG2.

Literature Information

  • ·Literature 1

    • Title

    • Effects of peptide dimerization on pore formation: Antiparallel disulfide-dimerized magainin 2 analogue.

    • Reference

    • Biopolymers. 2001 Apr 5;58(4):437-446.

    • Author

    • Hara T, Kodama H, Kondo M, Wakamatsu K, Takeda A, Tachi T, Matsuzaki K.