• • DRAMP ID

  • DRAMP02272

• • Peptide Name

  • PGLa (chain of PYLa/PGLa A; Frogs, amphibians, animals)

• • Source

  • Xenopus laevis (African clawed frog)

• • Family

  • Belongs to the gastrin/cholecystokinin family (Magainin subfamily)

• • Gene

  • pgla-a AND pgla-b

• • Sequence

  • GMASKAGAIAGKIAKVALKAL

• • Sequence Length

  • 21

• • UniProt Entry

  • Q99134,B7ZSG6,Q91826

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • Gram-negative bacteria: Escherichia coli 25922 (MIC=62-125 µg/ml), Klebsiella pneumoniae 13883 (MIC=250-500 µg/ml), Pseudomonas aeruginosa 27853 (MIC=250-500 µg/ml);
    • Gram-positive bacteria: Staphylococcus aureus 29213 (MIC=125-500 µg/ml), Strepococcus faecalis (MIC>500 µg/ml).
    • Yeast: Candida albicans 14053 (MIC=250-500 µg/ml).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP02272.

DRAMP02272

• • Function

  • PGLa display a broad-spectrum of antibacterial activity against a range of Gram-positive and Gram-negative bacteria. PGLa also displays antifungal activity against C. albicans ATCC 14053.

• • Tissue specificity

  • Expressed by the skin glands. Synthesized in the stomach and stored in a novel granular multinucleated cell in the gastric mucosa. Stored as active, processed peptides in large granules within the granular gland secretions of the skin.

• • PTM

  • Leucine amide at position 21.

• ·Literature 1

• • Title

  • Biosynthesis and degradation of peptides derived from Xenopus laevis prohormones.

• • Pubmed ID

  • 3606567

• • Reference

  • Biochem J. 1987 Apr 1;243(1):113-120.

• • Author

  • Giovannini MG, Poulter L, Gibson BW, Williams DH.

• ·Literature 2

• • Title

  • Antimicrobial peptides in the stomach of Xenopus laevis.

• • Pubmed ID

  • 1717472

• • Reference

  • J Biol Chem. 1991 Oct 15;266(29):19851-19857.

• • Author

  • Moore KS, Bevins CL, Brasseur MM, Tomassini N, Turner K, Eck H, Zasloff M.