General Information

    • DRAMP ID

    • DRAMP02308

    • Peptide Name

    • Signiferin-2.1 (Frogs, amphibians, animals)

    • Source

    • Crinia signifera (Common eastern froglet)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IIGHLIKTALGMLGL

    • Sequence Length

    • 15

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Bacillus cereus (MIC=25 µM), Enterococcus faecalis ATCC 29212 (MIC=100 µM), Leuconostoc lactis (MIC=12 µM), Listeria innocua (MIC=50 µM), Micrococcus luteus ATCC 9341 (MIC=25 µM), Staphylococcus aureus ATCC 29213 (MIC=12 µM), Staphylococcus epidermidis ATCC14990 (MIC=25 µM), Streptococcus uberis (MIC=25 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02308 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02308.

Physicochemical Information

    • Formula

    • C72H128N18O17S

    • Absent Amino Acids

    • CDEFNPQRSVWY

    • Common Amino Acids

    • L

    • Mass

    • 1549.98

    • PI

    • 8.76

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • 28.64

    • Hydrophobicity

    • 1.56

    • Aliphatic Index

    • 188.67

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP02308

Comments Information

    • Function

    • Has antibacterial activity against a wide spectrum of Gram-positive bacteria. Lacks antibacterial activity against the Gram-negative bacteria E. cloacae and E. coli. Inhibits the formation of NO by neuronal nitric oxide synthase with an IC50 of 12.4 µM.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Host-defence skin peptides of the Australian Common Froglet Crinia signifera: sequence determination using positive and negative ion electrospray mass spectra.

    • Reference

    • Rapid Commun Mass Spectrom. 2004;18(18):2155-2161.

    • Author

    • Maselli VM, Brinkworth CS, Bowie JH, Tyler MJ.
  • ·Literature 2

    • Title

    • Host-defence skin peptides of the Australian Streambank Froglet Crinia riparia: isolation and sequence determination by positive and negative ion electrospray mass spectrometry.

    • Reference

    • Rapid Commun Mass Spectrom. 2006;20(5):797-803.

    • Author

    • Maselli VM, Bilusich D, Bowie JH, Tyler MJ.
  • ·Literature 3

    • Title

    • Disulfide-containing peptides from the glandular skin secretions of froglets of the genus Crinia: structure, activity and evolutionary trends.

    • Reference

    • Regul Pept. 2008 Nov 29;151(1-3):80-87.

    • Author

    • Jackway RJ, Pukala TL, Maselli VM, Musgrave IF, Bowie JH, Liu Y, Surinya-Johnson KH, Donnellan SC, Doyle JR, Llewellyn LE, Tyler MJ.