General Information

    • DRAMP ID

    • DRAMP02317

    • Peptide Name

    • Chrysophsin-3 (fish, chordates, animals)

    • Source

    • Chrysophrys major (Red sea bream)

    • Family

    • Belongs to the pleurocidin family

    • Gene

    • Not found

    • Sequence

    • FIGLLISAGKAIHDLIRRRH

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.12581207]Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC=0.25 µM), Lactococcus garvieae ATCC 49156 (MIC=10 µM), S.iniae F-8502 (MIC=10 µM);
      • Gram-negative bacteria: Escherichia coli WT-2 (MIC=0.25 µM), Vibrio anguillarum ATCC 19264 (MIC=10 µM), Vibriopenaeicida KHA (MIC=10 µM), V. harveyi ATCC 14126 (MIC=5 µM), V. vulnificus ATCC 33148 (MIC=10 µM), Aeromonas salmonicida NCMB 1102 (MIC=10 µM), Pseudomonas putida ATCC 12633 (MIC=40 µM).

    • Hemolytic Activity

      • [Ref.12581207]0% hemolytic activity at 0.25 μM, 10% hemolytic activity at 1 μM against human erythrocytes.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02317 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C104H176N34O24

    • Absent Amino Acids

    • CEMNPQTVWY

    • Common Amino Acids

    • I

    • Mass

    • 2286.75

    • PI

    • 11.71

    • Basic Residues

    • 6

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +5

    • Boman Index

    • -28.83

    • Hydrophobicity

    • 0.345

    • Aliphatic Index

    • 146.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP02317

DRAMP02317 chydropathy plot

Comments Information

    • Function

    • Shows hemolytic activity human red blood cells.

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major.

    • Reference

    • Eur J Biochem. 2003 Feb;270(4):675-686.

    • Author

    • Iijima N, Tanimoto N, Emoto Y, Morita Y, Uematsu K, Murakami T, Nakai T.