General Information

    • DRAMP ID

    • DRAMP02320

    • Peptide Name

    • Grammistin PpIIb (Group II grammistin; fish, chordates, animals)

    • Source

    • Pogonoperca punctata (Clown grouper) (Bearded grouper)

    • Family

    • Belongs to the grammistin family (Group 2 subfamily)

    • Gene

    • Not found

    • Sequence

    • FIGGIISFIKKLF

    • Sequence Length

    • 13

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.Fish. Sci. 2001 Feb;67:163-169.]Gram-positive bacteria: Bacillus subtilis IAM 1026 (MIC=6.25 µg/ml), Staphylococcus aureus IAM 1011 (MIC=3.13 µg/ml);
      • Gram-negative bacteria: Aeromonas salmonicida NCIBM 1102 (MIC>50 µg/ml), Escherichia coli JCM 1649 (MIC=50 µg/ml), P. dameselae subsp. Piscicida Sp 9587 (MIC=12.5 µg/ml), Salmonella typhimurium S1 (MIC>50 µg/ml), S. putrefaciens IAM 1509 (MIC=6.25 µg/ml), Vibrio anguillarum NCIBM 829 (MIC=12.5 µg/ml), Vibrio parahaemolyticus (MIC=25 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Phospholipids

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02320 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C76H119N15O15

    • Absent Amino Acids

    • ACDEHMNPQRTVWY

    • Common Amino Acids

    • I

    • Mass

    • 1482.87

    • PI

    • 10

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • 20.92

    • Hydrophobicity

    • 1.6

    • Aliphatic Index

    • 150

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP02320

Comments Information

    • Function

    • Has antibacterial activities.

Literature Information

  • ·Literature 1

    • Title

    • Primary and secondary structures of grammistins, peptide toxins isolated from the skin secretion of the soapfish Pogonoperca punctata.

    • Reference

    • Fish. Sci. 2001 Feb;67:163-169.

    • Author

    • Shiomi K, Yokota H, Nagashima Y, Ishida M.