• • DRAMP ID

  • DRAMP02330

• • Peptide Name

  • Piscidin-1 (Pis-1; Piscidin 1; fish, chordates, animals)

• • Source

  • Morone saxatilis (Striped bass)

• • Family

  • Belongs to the pleurocidin family

• • Gene

  • Not found

• • Sequence

  • FFHHIFRGIVHVGKTIHRLVTG

• • Sequence Length

  • 22

• • UniProt Entry

  • Q8UUG0

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.11713517]Gram-positive bacteria: Enterococcus faecalis VRE (MIC=5-10 µM), E. faecalis (MIC=2.5-5 µM), Listeria monocytogenes (MIC=2.5-5 µM), Micrococcus luteus (MIC=10-20 µM), Staphylococcus aureus MRSA (MIC=1.25-2.5 µM), S. epidermitis (MIC=5-10 µM), S. saprophiticus (MIC=5-10 µM), Staphylococcus xylosus (MIC>20 µM), S. agalactiae (MIC=1.25-2.5 µM), S. bovis (MIC=1.25-2.5 µM), S. equisimilis (MIC=2.5-5 µM), S. mitis (MIC=1.25-2.5 µM), S. Pneumonae (MIC=1.25-2.5 µM), S. pyrogenes (MIC=1.25-2.5 µM), Streptococcus iniae KST740 ak (MIC=1.25-2.5 µM), S. iniae KSTSi 6P (MIC=1.25-2.5 µM);
    • Gram-negative bacteria: Aeromonas hydrophila (MIC>20 µM), Burkholderia cepacia (MIC>20 µM), Vibrio Cholera (MIC=2.5-5 µM), Escherichia coli (MIC=5-10 µM), Enterobacter cloacae (MIC=10-20 µM), E. Aerogenes (MIC=10-20 µM), Klebsiella pneumoniae (MIC=2.5-5 µM), K. oxytoca (MIC=5-10 µM), Salmonella choleraesuis (MIC=10-20 µM), S. typhimurium (MIC=10-20 µM), S. arizonae (MIC=10-20 µM), Serratia marcescens (MIC>20 µM), Shigella flexneri (MIC=2.5-5 µM), S. sonnei (MIC=5-10 µM), Yersinia enterocolitica (MIC=2.5-5 µM); Fungi: Neurospora crassa (MIC=1.56-3.12 µM), A. fumigatus (MIC=50-100 µM), F. axysporum (MIC=0.78-1.56 µM), F. culmorum (MIC=0.39-0.78 µM), C. ablicans (MIC=10-20 µM), C. glabrata (MIC=10-20 µM), C. lusitania (MIC=10-20 µM), C. tropacalis (MIC=10-20 µM).

• • Hemolytic Activity

  • • [Ref.11713517]10% hemolytic activity at 5 μg/mL, 60% hemolytic activity at 10 μg/mL, 100% hemolytic activity at 50 μg/mL against human erythrocytes

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 2JOS,2OJM resolved by NMR.
• 2JOS-> 

• Predicted Structure

• There is no predicted structure for DRAMP02330.

DRAMP02330

• • Function

  • Exhibits broad spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria. Also has hemolytic activity. Seems to disrupt the membranes by adopting an alpha helical conformation and forming toroidal pores.

• ·Literature 1

• • Title

  • Peptide antibiotics in mast cells of fish.

• • Pubmed ID

  • 11713517

• • Reference

  • Nature. 2001 Nov 15;414(6861):268-269.

• • Author

  • Silphaduang U, Noga EJ.