• • DRAMP ID

  • DRAMP02331

• • Peptide Name

  • Piscidin-2 (Pis-2; fish, chordates, animals)

• • Source

  • Morone chrysops (White bass)

• • Family

  • Belongs to the pleurocidin family

• • Gene

  • Not found

• • Sequence

  • FFHHIFRGIVHVGKTIHKLVTG

• • Sequence Length

  • 22

• • UniProt Entry

  • Q8UUG2

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • Gram-positive bacteria: Enterococcus faecalis (MIC=5-10 µM), E. faecalis (MIC=2.5-5 µM), Listeria monocytogenes (MIC=2.5-5 µM), Micrococcus luteus (MIC=10-20 µM), Staphylococcus aureus (MIC=1.25-2.5 µM), S. epidermidis (MIC=5-10 µM), S. saprophiticus (MIC=5-10 µM), S. xylosus (MIC>20 µM), Streptococcus agalactiae (MIC=1.25-2.5 µM), S. bovis (MIC=1.25-2.5 µM), S. equisimilis (MIC=2.5-5 µM), S. mitis (MIC=1.25-2.5 µM), Streptococcus pneumoniae (MIC=1.25-2.5 µM), Streptococcus pyogenes (MIC=1.25-2.5 µM), S. iniae KST740ak (MIC=1.25-2.5 µM), S. iniae KSTSi 6P (MIC=1.25-2.5 µM);
    • Gram-negative bacteria: Aeromonas hydrophila (MIC>20 µM), Burkholderia cepacia (MIC>20 µM), Vibrio cholera (MIC=2.5-5 µM), Escherichia coli (MIC=5-10 µM), Moraxella catarrhalis (MIC=2.5-5 µM), Neisseria gonorrhoeae (MIC>20 µM), Pseudomonas aeruginosa (MIC=5-10 µM), Enterobacter cloacae (MIC=10-20 µM), E. aerogenes (MIC=10-20 µM), Klebsiella pneumoniae (MIC=2.5-5 µM), K. oxytoca (MIC=5-10 µM), Salmonella choleraesuis (MIC=10-20 µM), Salmonella typhimurium (MIC=10-20 µM), S. arizonae (MIC=10-20 µM), Serratia marcescens (MIC>20 µM), Shigella flexneri (MIC=2.5-5 µM), S. sonnei (MIC=5-10 µM), Yersinia enterocolitica (MIC=2.5-5 µM).
    • Fungi: Neurospora crassa (MIC=1.56-3.12 µM), Aspergillus fumigatus (MIC=50-100 µM), Fusarium oxysporum (MIC=0.78-1.56 µM), F. culmorum (MIC=0.39-0.78 µM), Candida albicans (MIC=10-20 µM), C. glabrata (MIC=10-20 µM), C. lusitania (MIC=10-20 µM), C. tropicalis (MIC=10-20 µM).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP02331.

DRAMP02331

• • Function

  • Exhibits broad spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria as well as against a variety of fungi. Seems to disrupt the membranes by adopting an alpha helical conformation.

• • Tissue specificity

  • Expressed in gill, skin, intestine, spleen, anterior kidney, and blood cells.

• ·Literature 1

• • Title

  • Discovery and characterization of two isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass.

• • Pubmed ID

  • 11739390

• • Reference

  • J Biol Chem. 2002 Feb 15;277(7):5030-5039.

• • Author

  • Lauth X, Shike H, Burns JC, Westerman ME, Ostland VE, Carlberg JM, Van Olst JC, Nizet V, Taylor SW, Shimizu C, Bulet P.