General Information

    • DRAMP ID

    • DRAMP02350

    • Peptide Name

    • Pleurocidin (NRC-4; fish, chordates, animals)

    • Source

    • Pseudopleuronectes americanus (Winter flounder 2-2.1)

    • Family

    • Belongs to the pleurocidin family

    • Gene

    • ple1 AND ple2

    • Sequence

    • GWGSFFKKAAHVGKHVGKAALTHYL

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Escherichia coli (MIC=2.2-3.3 µM), Leucothrix mucor (MIC>35.0 µM), Serratia marcescens (MIC>35.0 µM), Pseudomonas aeruginosa (MIC>35.0 µM), Aeromonas salmonicida (MIC=17.7-35.0 µM), Cytophaga aquatilis (MIC=2.2-4.4 µM), Pasteurella haemolytica (MIC=4.4-8.8 µM), Salmonella typhimurium I (MIC=8.8-17.7 µM), Salmonella typhimurium II (MIC=8.8-17.7 µM);
      • Gram-positive bacteria: Bacillus subtilis (MIC=1.1-2.2 µM), Staphylococcus aureus (MIC=17.7-35.0 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix (1 helices; 19 residues)

    • Structure Description

    • From NMR structural studies with the uniformly (15)N-labeled peptide, a structure of pleurocidin is determined to be in a random coil conformation in aqueous solution whereas it assumes an alpha-helical structure in TFE and in dodecylphosphocholine (DPC) micelles.(Ref.2)

    • Helical Wheel Diagram

    • DRAMP02350 helical wheel diagram

    • PDB ID

    • 1Z64  resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP02350.

Physicochemical Information

    • Formula

    • C129H192N36O29

    • Absent Amino Acids

    • CDEIMNPQR

    • Common Amino Acids

    • AGK

    • Mass

    • 2711.17

    • PI

    • 10.18

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +7

    • Boman Index

    • -5.08

    • Hydrophobicity

    • -0.068

    • Aliphatic Index

    • 70.4

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 291.25

    • Polar Residues

    • 7

DRAMP02350

DRAMP02350 chydropathy plot

Comments Information

    • May play a role in innate host defense.

Literature Information

  • ·Literature 1

    • Title

    • Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder.

    • Reference

    • J Biol Chem. 1997 May 2;272(18):12008-12013.

    • Author

    • Cole A.M, Weis P, Diamond G.