General Information

    • DRAMP ID

    • DRAMP02358

    • Peptide Name

    • Pleurocidin-like peptide WF4 (NRC-6; fish, chordates, animals)

    • Source

    • Pseudopleuronectes americanus (Winter flounder 4-1.1)

    • Family

    • Belongs to the pleurocidin family

    • Gene

    • ple4

    • Sequence

    • GWGSIFKHGRHAAKHIGHAAVNHYL

    • Sequence Length

    • 25

    • Protein Existence

    • Homology

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Aeromonas salmonicida 99-1 (MIC=4 µg/ml), A. salmonicida 97-4 (MIC=4 µg/ml), Salmonella enterica serovar Typhimurium MS7953s (MIC=4 µg/ml), S. enterica serovar Typhimurium 14028s (MIC=64 µg/ml), Pseudomonas aeruginosa K799 (MIC=16 µg/ml), Pseudomonas aeruginosa Z61 (MIC=4 µg/ml), Escherichia coli CGSC 4908 (MIC=4 µg/ml), E. coli UB1005 (MIC=4 µg/ml), E. coli DC2 (MIC=2 µg/ml);
      • Gram-positive bacteria: Staphylococcus epidermidis C621 (MIC>64 µg/ml), Staphylococcus aureus (MRSA) C623 (MIC=32 µg/ml).
      • Yeast: Candida albicans C627 (MIC=32 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02358 helical wheel diagram

    • Predicted Structure

Physicochemical Information

    • Formula

    • C127H186N42O29

    • Absent Amino Acids

    • CDEMPQT

    • Common Amino Acids

    • H

    • Mass

    • 2765.14

    • PI

    • 10.29

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +8

    • Boman Index

    • -24.39

    • Hydrophobicity

    • -0.376

    • Aliphatic Index

    • 74.4

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 291.25

    • Polar Residues

    • 7

DRAMP02358

DRAMP02358 chydropathy plot

Comments Information

    • Comment

    • No comments found on DRAMP database

Literature Information

  • ·Literature 1

    • Title

    • Novel antimicrobial peptides derived from flatfish genes.

    • Reference

    • Antimicrob Agents Chemother. 2003 Aug;47(8):2464-2470.

    • Author

    • Patrzykat A, Gallant JW, Seo JK, Pytyck J, Douglas SE.
  • ·Literature 2

    • Title

    • Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum).

    • Reference

    • Dev Comp Immunol. 2001 Mar;25(2):137-147.

    • Author

    • Douglas SE, Gallant JW, Gong Z, Hew C.