• • DRAMP ID

  • DRAMP02374

• • Peptide Name

  • Bass hepcidin (fish, chordates, animals)

• • Source

  • Morone chrysops x Morone saxatilis (white bass x striped sea-bass)

• • Family

  • Belongs to the hepcidin family

• • Gene

  • hamp

• • Sequence

  • GCRFCCNCCPNMSGCGVCCRF

• • Sequence Length

  • 21

• • UniProt Entry

  • P82951

• • Protein Existence

  • Protein level

• • SMILES

  • CSCC[C@@H]1NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H]2CCCN2C(=O)[C@@H]2CSSC[C@@H]3NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CSSC[C@@H]4NC(=O)[C@H](CSSC[C@H](NC(=O)[C@H](CC(N)=O)NC4=O)C(=O)N2)NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)CN)CSSC[C@@H](C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc2ccccc2)C(=O)O)NC3=O)NC(=O)CNC(=O)[C@H](CO)NC1=O

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-, Antifungal

• • Target Organism

  • • Gram-negative bacteria:

      Target Organism	Activity
      Escherichia coli ATCC 25	-
      922	-
      351	-
      50	MIC=22 µM
      Escherichia coli ATCC D31K	MIC=11 µM
      Klebsiella pneumoniae ATCC 10	-
      031	MIC=22 µM
      P. shigelloides ATCC KST	MIC=11 µM
      S. flexneri ATCC 12	-
      022	MIC=22 µM
      Shigella sonnei ATCC 9290	MIC=44 µM
      Y. enterocolitica ATCC 23	-
      715	MIC=22 µM

    • Fungi:

      Target Organism	Activity
      Aspergillus niger	MIC=44 µM

• • Hemolytic Activity

  • • [Ref.15546886] It displays essentially no hemolytic activity toward HSB erythrocytes

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bonds between Cys2 and Cys19; Cys5 and Cys8; Cys6 and Cys15,Cys9 and Cys18.

• • Stereochemistry

  • L

• • PDB ID

  • 1S6W resolved by NMR.

• Predicted Structure

• Please click DRAMP02374_predicted_structure.pdb to download.

DRAMP02374

• • Function

  • Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages (By similarity). Synthetic bass hepcidin is active in vitro against Gram-negative pathogens and fungi but showes no activity against key Gram-positive pathogens and a single yeast strain tested.

• • Tissue specificity

  • Predominantly expressed in liver.

• • Induction

  • By bacterial challenge.

• • PTM

  • Contains four disulfide bonds 2-19; 5-8; 6-15; 9-18.

• ·Literature 1

• • Title

  • Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge.

• • Pubmed ID

  • 11985602

• • Reference

  • Eur J Biochem. 2002 Apr;269(8):2232-2237.

• • Author

  • Shike H, Lauth X, Westerman ME, Ostland VE, Carlberg JM, Van Olst JC, Shimizu C, Bulet P, Burns JC.

• ·Literature 2

• • Title

  • Bass hepcidin synthesis, solution structure, antimicrobial activities and synergism, and in vivo hepatic response to bacterial infections.

• • Pubmed ID

  • 15546886

• • Reference

  • J Biol Chem. 2005 Mar 11;280(10):9272-9282.

• • Author

  • Lauth X, Babon JJ, Stannard JA, Singh S, Nizet V, Carlberg JM, Ostland VE, Pennington MW, Norton RS, Westerman ME.