General Information

    • DRAMP ID

    • DRAMP02380

    • Peptide Name

    • Grammistin Gs C (Group III grammistin; soapfish, chordates, animals)

    • Source

    • Grammistes sexlineatus (Sixline soapfish) (Golden stripe soapfish)

    • Family

    • Belongs to the grammistin family (Group 3 subfamily)

    • Gene

    • Not found

    • Sequence

    • NWRKILGKIAKVAAGLLGSMLAGYQV

    • Sequence Length

    • 26

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Bacillus subtilis IAM 1026 (MIC=6.25 µg/ml), Staphylococcus aureus IAM 1011 (MIC=12.5 µg/ml);
      • Gram-negative bacteria: Aeromonas salmonicida NCIBM 1102 (MIC=25 µg/ml), Escherichia coli JCM 1649 (MIC=12.5 µg/ml), P. dameselae subsp. Piscicida Sp 9587 (MIC=6.25 µg/ml), Salmonella typhimurium S1 (MIC=12.5 µg/ml), S. putrefaciens IAM 1509 (MIC=6.25 µg/ml), Vibrio anguillarum NCIBM 829 (MIC=12.5 µg/ml), Vibrio parahaemolyticus (MIC=25 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02380 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02380.

Physicochemical Information

    • Formula

    • C127H213N35O31S

    • Absent Amino Acids

    • CDEFHPT

    • Common Amino Acids

    • AGL

    • Mass

    • 2758.36

    • PI

    • 10.46

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • 5.99

    • Hydrophobicity

    • 0.535

    • Aliphatic Index

    • 127.69

    • Half Life

      • Mammalian:1.4 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 279.6

    • Polar Residues

    • 7

DRAMP02380

DRAMP02380 chydropathy plot

Comments Information

    • MOA

    • Thanks to its amphiphilic alpha-helice(s), it may integrate into membrane phospholipids, leading to lysis of the membrane.

Literature Information

  • ·Literature 1

    • Title

    • Further isolation and characterization of grammistins from the skin secretion of the soapfish Grammistes sexlineatus.

    • Reference

    • Toxicon. 2005 Apr;45(5):595-601.

    • Author

    • Sugiyama N, Araki M, Ishida M, Nagashima Y, Shiomi K.