• • DRAMP ID

  • DRAMP02407

• • Peptide Name

  • Napin-like polypeptide (Contains: Napin-like polypeptide small chain and large chain)

• • Source

  • Brassica oleracea var. alboglabra (Chinese kale) (Brassicaalboglabra)

• • Family

  • Belongs to the 2S seed storage albumins family

• • Gene

  • Not found

• • Sequence

  • PAQPFRIKKRQGPFERP

• • Sequence Length

  • 17

• • UniProt Entry

  • P84782

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • Gram-negative bacterium: Pseudomonas fluorescens (IC50=89±6.1 µM);
    • Gram-positive bacteria: Bacillus subtilis (IC50=278±12.9 µM), B. cereus (IC50=145±83 µM), Bacillus megaterium (IC50=156±8.4 µM), Megabacterium phlei (IC50=104±27 µM).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP02407.

DRAMP02407

• • Function

  • Inhibits cell-free protein synthesis. Inhibits trypsin and chymotrypsin. Does not inhibit ribonuclease of protease activity. Has antibacterial activity except Gram-positive bacterium S. aureus or the Gram-negative bacteria E. coli, E. aerogenes, P. aeruginosa and P. vulgaris. Inhibits the proliferation of leukemia cells in vitro.

• • Subunit structure

  • The mature protein consists of a small and a large chain linked by disulfide bonds (sequence shown is small chain).

• • Biophysicochemical properties

  • This activity is preserved between pH 5 and pH 11, and between 10 and 40 degrees C. It falls to a low level at pH 3 and pH 13 and at 70 degrees C.

• ·Literature 1

• • Title

  • A napin-like polypeptide with translation-inhibitory, trypsin-inhibitory, antiproliferative and antibacterial activities from kale seeds.

• • Pubmed ID

  • 15485558

• • Reference

  • J Pept Res. 2004 Nov;64(5):202-208.

• • Author

  • A napin-like polypeptide with translation-inhibitory, trypsin-inhibitory, antiproliferative and antibacterial activities from kale seeds.