• DRAMP ID

    • DRAMP02460
    • Peptide Name

    • L-amino-acid oxidase (LAAO; LAO; snakes, reptils, animals)
    • Source

    • Bothrops leucurus (White-tailed jararaca) (White-tailed lancehead)
    • Family

    • Belongs to the flavin monoamine oxidase family (FIG1 subfamily)
    • Gene

    • Unknown
    • Sequence

    • ADDRNPLEECFRETDYEEFLEIAKNGLSTT
    • Sequence Length

    • 30
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Antiparasitic, Antimicrobial
    • Target Organism

      • No MICs found on DRAMP database
    • Hemolytic activity

    • Unknown
    • Binding Target

    • Unknown
    • Structure

    • Unknown
    • Structure Description

    • Unknown
    • DRAMP02460 helical wheel diagram
    • Formula

    • C150H229N39O56S
    • Absent Amino Acids

    • HMQVW
    • Common Amino Acids

    • E
    • Mass

    • 4024.2
    • PI

    • 3.81
    • Basic Residues

    • 3
    • Acidic Residues

    • 9
    • Hydrophobic Residues

    • 8
    • Boman Index

    • -95.46
    • Hydrophobicity

    • -100
    • Aliphatic Index

    • 58.67
    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 1490
    • Absorbance 280nm

    • 51.38
    • Polar Residues

    • 9

DRAMP02460

DRAMP02460 chydropathy plot
    • Function

    • Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (high activity against L-Met, L-Leu, L-Nle, L-Trp, L-Phe and moderate activity against L-Tyr), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, antibacterial and antiparasitic activities (By similarity). In addition, this protein induces apoptosis. It also interacts with endothelial cells, and inhibits collagen- and ADP-induced platelet aggregation. L-LAAO family effects on platelets are controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions.
    • Tissue specificity

    • Expressed by the venom gland.
    • Miscellaneous

    • Has parasiticidal activities against both trypanosomes and leishmania, as a result of enzyme-catalyzed hydrogen peroxide production.
    • Biophysicochemical properties

    • pH dependence (Optimum pH is 7.5-8.8 for L-Leu).
  • Literature 1
    • Reference

    • Biochim Biophys Acta. 2011 Jul;1810(7):683-694.
    • Author

    • Naumann GB, Silva LF, Silva L, Faria G, Richardson M, Evangelista K, Kohlhoff M, Gontijo CM, Navdaev A, de Rezende FF, Eble JA, Sanchez EF.
    • Title

    • Cytotoxicity and inhibition of platelet aggregation caused by an l-amino acid oxidase from Bothrops leucurus venom.