General Information

    • DRAMP ID

    • DRAMP02549

    • Peptide Name

    • CRISPR-associated endoribonuclease Cas2 (Antiviral defensin)

    • Source

    • Escherichia coli (strain K12)

    • Family

    • Belongs to the CRISPR-associated endoribonuclease Cas2 prote

    • Gene

    • ygbF

    • Sequence

    • MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTPVDLDGLRLVSFLPV

    • Sequence Length

    • 94

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Metal-binding

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02549 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02549.

Physicochemical Information

    • Formula

    • C475H758N128O133S4

    • Absent Amino Acids

    • CH

    • Common Amino Acids

    • V

    • Mass

    • 10518.26

    • PI

    • 5.02

    • Basic Residues

    • 9

    • Acidic Residues

    • 11

    • Hydrophobic Residues

    • 42

    • Net Charge

    • -2

    • Boman Index

    • -94.13

    • Hydrophobicity

    • 0.279

    • Aliphatic Index

    • 109.79

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 17990

    • Absorbance 280nm

    • 193.44

    • Polar Residues

    • 22

DRAMP02549

DRAMP02549 chydropathy plot

Comments Information

    • Function

    • CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).

    • Induction

    • Repressed by H-NS, activated by LeuO. Activated by the BaeSR two-component regulatory system, possibly due to envelope stress. Part of the casABCDE-ygbT-ygbF operon.

Literature Information

  • ·Literature 1

    • Title

    • Unusual nucleotide arrangement with repeated sequences in the Escherichia coli K-12 chromosome.

    • Reference

    • J Bacteriol. 1989 Jun;171(6):3553-6.

    • Author

    • Nakata A, Amemura M, Makino K.
  • ·Literature 2

    • Title

    • The complete genome sequence of Escherichia coli K-12.

    • Reference

    • Science. 1997 Sep 5;277(5331):1453-1462.

    • Author

    • Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y.
  • ·Literature 3

    • Title

    • Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.

    • Reference

    • Mol Syst Biol. 2006;2:2006.0007.

    • Author

    • Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T.