General Information

    • DRAMP ID

    • DRAMP02559

    • Peptide Name

    • CRISPR-associated endoribonuclease Cas2 (Antiviral defensin)

    • Source

    • Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 /JCM 10044 / NBRC 100330 / Delt

    • Family

    • Belongs to the CRISPR-associated endoribonuclease Cas2 prote

    • Gene

    • cas2

    • Sequence

    • MVVTVYLLIVYDVGVERVNRVKSYLRTELHWVQNSVFEGEVTESQFRRIETNLERIIDRERDSVIIYSFRSERAMNRNVLGLEKSPLDVIL

    • Sequence Length

    • 91

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Metal-binding

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02559 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02559.

Physicochemical Information

    • Formula

    • C481H778N136O142S2

    • Absent Amino Acids

    • C

    • Common Amino Acids

    • V

    • Mass

    • 10802.41

    • PI

    • 5.83

    • Basic Residues

    • 14

    • Acidic Residues

    • 14

    • Hydrophobic Residues

    • 35

    • Net Charge

    • 0

    • Boman Index

    • -205.9

    • Hydrophobicity

    • -0.141

    • Aliphatic Index

    • 114.29

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 11460

    • Absorbance 280nm

    • 127.33

    • Polar Residues

    • 23

DRAMP02559

DRAMP02559 chydropathy plot

Comments Information

    • Function

    • CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).

Literature Information

  • ·Literature 1

    • Title

    • Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.

    • Reference

    • J Bacteriol. 1997 Nov;179(22):7135-7155.

    • Author

    • Smith DR, Doucette-Stamm LA, Deloughery C, Lee H, Dubois J, Aldredge T, Bashirzadeh R, Blakely D, Cook R, Gilbert K, Harrison D, Hoang L, Keagle P, Lumm W, Pothier B, Qiu D, Spadafora R, Vicaire R, Wang Y, Wierzbowski J, Gibson R, Jiwani N, Caruso A, Bush D, Reeve JN, et al.
  • ·Literature 2

    • Title

    • A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats.

    • Reference

    • J Biol Chem. 2008 Jul 18;283(29):20361-71.

    • Author

    • Beloglazova N, Brown G, Zimmerman MD, Proudfoot M, Makarova KS, Kudritska M, Kochinyan S, Wang S, Chruszcz M, Minor W, Koonin EV, Edwards AM, Savchenko A, Yakunin AF