• DRAMP ID

    • DRAMP02741
    • Peptide Name

    • Pelovaterin (defensin-like AMP; Gly-rich; Reptiles, animals)
    • Source

    • Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • DDTPSSRCGSGGWGPCLPIVDLLCIVHVTVGCSGGFGCCRIG
    • Sequence Length

    • 42
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • Gram-negative bacteria: Pseudomonas aeruginosa (EC50=0.42 µg/mL), Proteus vulgaris (EC50=0.37 µg/mL), Proteus mirabilis (EC50=8.4 µg/mL);
      • Gram-positive bacterium: Staphylococcus aureus (EC50=42 µg/mL).
    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Combine helix and strand structure
    • Structure Description

    • An analysis of the ensemble of pelovaterin structures reveals that hydrophilic residues Asp1 Arg7 begin with a random coil structure leading to a short 310 helix between Cys8 and Gly12, whereas antiparallel â-strands are formed by the residues Leu17 Val20 (β), His27 Val28 (â2), and Gly34 Cys39 (â3) arranged in a βâ2â3 topology.
    • Helical Wheel Diagram

    • DRAMP02741 helical wheel diagram
    • PDB ID

    • 2JR3 resolved by NMR.
  • 2JR3-> 
    • Predicted Structure

    • There is no predicted structure for DRAMP02741.
    • Formula

    • C177H281N51O55S6
    • Absent Amino Acids

    • AEKMNQY
    • Common Amino Acids

    • G
    • Mass

    • 4195.85
    • PI

    • 5.3
    • Basic Residues

    • 3
    • Acidic Residues

    • 3
    • Hydrophobic Residues

    • 12
    • Net Charge

    • 0
    • Boman Index

    • -12.27
    • Hydrophobicity

    • 0.545
    • Aliphatic Index

    • 83.33
    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5875
    • Absorbance 280nm

    • 143.29
    • Polar Residues

    • 21

DRAMP02741

DRAMP02741 chydropathy plot
    • Function

    • Induces the nucleation and stabilization of vaterite, one of the crystalline polymorphs of calcium carbonate. Exhibits strong antimicrobial activity against Pseudomonas aeruginosa and Proteus vulgaris.
    • PTM

    • Contains three disulfide bonds 8-38; 16-32; 24-39.
  • ·Literature 1
    • Title

    • Purification and characterization of a vaterite-inducing peptide, pelovaterin, from the eggshells of Pelodiscus sinensis (Chinese soft-shelled turtle).
    • Reference

    • Biomacromolecules. 2005 May-Jun;6(3):1429-37.
    • Author

    • Lakshminarayanan R, Chi-Jin EO, Loh XJ, Kini RM, Valiyaveettil S.
  • ·Literature 2
    • Title

    • Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix.
    • Reference

    • J Am Chem Soc. 2008 Apr 9;130(14):4660-8.
    • Author

    • minarayanan R, Vivekanandan S, Samy RP, Banerjee Y, Chi-Jin EO, Teo KW, Jois SD, Kini RM, Valiyaveettil S.