• • DRAMP ID

  • DRAMP02741

• • Peptide Name

  • Pelovaterin (defensin-like AMP; Gly-rich; Reptiles, animals)

• • Source

  • Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • DDTPSSRCGSGGWGPCLPIVDLLCIVHVTVGCSGGFGCCRIG

• • Sequence Length

  • 42

• • UniProt Entry

  • P84818

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • Gram-negative bacteria: Pseudomonas aeruginosa (EC50=0.42 µg/mL), Proteus vulgaris (EC50=0.37 µg/mL), Proteus mirabilis (EC50=8.4 µg/mL);
    • Gram-positive bacterium: Staphylococcus aureus (EC50=42 µg/mL).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Combine helix and strand structure

• • Structure Description

  • An analysis of the ensemble of pelovaterin structures reveals that hydrophilic residues Asp1 Arg7 begin with a random coil structure leading to a short 310 helix between Cys8 and Gly12, whereas antiparallel â-strands are formed by the residues Leu17 Val20 (β), His27 Val28 (â2), and Gly34 Cys39 (â3) arranged in a βâ2â3 topology.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 2JR3 resolved by NMR.

• Predicted Structure

• There is no predicted structure for DRAMP02741.

DRAMP02741

• • Function

  • Induces the nucleation and stabilization of vaterite, one of the crystalline polymorphs of calcium carbonate. Exhibits strong antimicrobial activity against Pseudomonas aeruginosa and Proteus vulgaris.

• • PTM

  • Contains three disulfide bonds 8-38; 16-32; 24-39.

• ·Literature 1

• • Title

  • Purification and characterization of a vaterite-inducing peptide, pelovaterin, from the eggshells of Pelodiscus sinensis (Chinese soft-shelled turtle).

• • Pubmed ID

  • 15877362

• • Reference

  • Biomacromolecules. 2005 May-Jun;6(3):1429-37.

• • Author

  • Lakshminarayanan R, Chi-Jin EO, Loh XJ, Kini RM, Valiyaveettil S.

• ·Literature 2

• • Title

  • Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix.

• • Pubmed ID

  • 18341335

• • Reference

  • J Am Chem Soc. 2008 Apr 9;130(14):4660-8.

• • Author

  • minarayanan R, Vivekanandan S, Samy RP, Banerjee Y, Chi-Jin EO, Teo KW, Jois SD, Kini RM, Valiyaveettil S.