General Information

    • DRAMP ID

    • DRAMP02780

    • Peptide Name

    • Defensin-B (Defensin B; Insects, animals)

    • Source

    • Anomala cuprea (Cupreous chafer beetle)

    • Family

    • Belongs to the invertebrate defensin family (Type 1 subfamily)

    • Gene

    • Not found

    • Sequence

    • VTCDLLSFEAKGFAANHSICAAHCLVIGRKGGACQNGVCVCRN

    • Sequence Length

    • 43

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Bacillus megaterium (MIC=1.2-2.5 µM), Bacillus subtilis (MIC=20-40 µM), Micrococcus luteus (MIC=1.2-2.5 µM), Staphylococcus aureus (MIC=2.5-5 µM);
      • Gram-negative bacterium: X. japonicus (MIC=20-40 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys3 and Cys34; Cys20 and Cys39; Cys24 and Cys41.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02780 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02780.

Physicochemical Information

    • Formula

    • C186H303N59O55S6

    • Absent Amino Acids

    • MPWY

    • Common Amino Acids

    • AC

    • Mass

    • 4438.17

    • PI

    • 8.34

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 17

    • Net Charge

    • +4

    • Boman Index

    • -30.66

    • Hydrophobicity

    • 0.467

    • Aliphatic Index

    • 86.28

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 375

    • Absorbance 280nm

    • 8.93

    • Polar Residues

    • 17

DRAMP02780

DRAMP02780 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity against Gram-positive bacteria and the Gram-negative bacterium X. japonicus.

    • PTM

    • Problely contains three disulfide bonds 3-34; 20-39; 24-41.

Literature Information

  • ·Literature 1

    • Title

    • Two novel insect defensins from larvae of the cupreous chafer, Anomala cuprea: purification, amino acid sequences and antibacterial activity.

    • Reference

    • Insect Biochem Mol Biol. 2001 Dec;32(1):75-84.

    • Author

    • Yamauchi H.