General Information

    • DRAMP ID

    • DRAMP02802

    • Peptide Name

    • Paneth cell-specific alpha-defensin 1 (DEFA1; horse defensin; houses, mammals, animals

    • Source

    • Equus caballus (Horse)

    • Family

    • Not found

    • Gene

    • DEFA1

    • Sequence

    • AREASKSLIGTASCTCRRAWICRWGERHSGKCIDQKGSTYRLCCRR

    • Sequence Length

    • 46

    • Protein Existence

    • Predicted

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Escherichia coli [ATCC 11775 (MBC=0.4 µM); ATCC 25922 (MBC=0.4 µM); ATCC 35218 (MBC=0.4 µM)], E. coli D31 (MBC=0.8 µM), Pseudomonas aeruginosa ATCC 11440 (MBC=3 µM);
      • Gram-positive bacteria: Staphylococcus aureus [ATCC 6538 (MBC=0.8 µM); ATCC 12600 (MBC=1.5 µM)], Staphylococcus epidermidis ATCC 14990 (MBC=1.5 µM), Enterococcus faecalis ATCC 29212 (MBC=6.1 µM), E. faecalis PEG 205 (wild-type) (MBC=3 µM), Streptococcus pyogenes ATCC 12344 (MBC=0.8 µM), Bacillus megaterium ATCC 14581 (MBC=0.2 µM).
      • Yeast: Candida albicans ATCC 24433 (MIC=0.8 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys14 and Cys44; Cys16 and Cys32; Cys22 and Cys43.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02802 helical wheel diagram

    • PDB ID

    • 2MXQ resolved by NMR.
  • 2MXQ-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP02802.

Physicochemical Information

    • Formula

    • C217H360N78O63S6

    • Absent Amino Acids

    • FMNPV

    • Common Amino Acids

    • R

    • Mass

    • 5262.09

    • PI

    • 9.86

    • Basic Residues

    • 12

    • Acidic Residues

    • 3

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +9

    • Boman Index

    • -145.46

    • Hydrophobicity

    • -0.704

    • Aliphatic Index

    • 51.09

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 12865

    • Absorbance 280nm

    • 285.89

    • Polar Residues

    • 19

DRAMP02802

DRAMP02802 chydropathy plot

Comments Information

    • Function

    • Membrane permeabilization is at least an essential part of the peptide's mode of action.

Literature Information

  • ·Literature 1

    • Title

    • A novel horse alpha-defensin: gene transcription, recombinant expression and characterization of the structure and function.

    • Reference

    • Biochem J. 2007 Oct 15;407(2):267-276.

    • Author

    • Bruhn O, Regenhard P, Michalek M, Paul S, Gelhaus C, Jung S, Thaller G, Podschun R, Leippe M, Grötzinger J, Kalm E.