General Information

    • DRAMP ID

    • DRAMP02817

    • Peptide Name

    • Pyrrhocoricin

    • Source

    • Pyrrhocoris apterus (Sap sucking bug) (Cimex apterus)

    • Family

    • Belongs to the drosocin family

    • Gene

    • Not found

    • Sequence

    • VDKGSYLPRPTPPRPIYNRN

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Micrococcus luteus (MIC>80 µM in L.B, P.B), Bacillus megaterium (MIC>80 µM in L.B, P.B);
      • Gram-negative bacteria: Escherichia coliD22 (MIC=0.3 µM in L.B, P.B), A.tumefaciens (MIC=1.25 µM in LB and 3 µM in PB), Salmonella typhimurium (MIC=5 µM in L.B and 10 µM in P.B).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02817 helical wheel diagram

    • PDB ID

    • 4EZN resolved by X-ray.
  • 4EZN-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C105H166N32O29

    • Absent Amino Acids

    • ACEFHMQW

    • Common Amino Acids

    • P

    • Mass

    • 2340.67

    • PI

    • 10.27

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 3

    • Net Charge

    • +3

    • Boman Index

    • -63.74

    • Hydrophobicity

    • -1.395

    • Aliphatic Index

    • 53.5

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 2980

    • Absorbance 280nm

    • 156.84

    • Polar Residues

    • 7

DRAMP02817

DRAMP02817 chydropathy plot

Comments Information

    • Function

    • Antibacterial peptide. Affects Gram-negative bacteria E.coli 1106, P.aeruginosa, E.coli D22 and E.cloacae and Gram-positive bacteria M.luteus and B.subtilis.

Literature Information

  • ·Literature 1

    • Title

    • Range of activity and metabolic stability of synthetic antibacterial glycopeptides from insects.

    • Reference

    • Biochim Biophys Acta. 1999 Feb 2;1426(3):459-467.

    • Author

    • Hoffmann R, Bulet P, Urge L, Otvös L Jr.
  • ·Literature 2

    • Title

    • Novel inducible antibacterial peptides from a hemipteran insect, the sap-sucking bug Pyrrhocoris apterus.

    • Reference

    • Biochem J. 1994 Jun 1;300 (Pt 2):567-575.

    • Author

    • Cociancich S, Dupont A, Hegy G, Lanot R, Holder F, Hetru C, Hoffmann JA, Bulet P.
  • ·Literature 3

    • Title

    • Structural Studies on the Forward and Reverse Binding Modes of Peptides to the Chaperone DnaK.

    • Reference

    • J Mol Biol. 2013 Jul 24;425(14):2463-2479.

    • Author

    • Zahn M, Berthold N, Kieslich B, Knappe D, Hoffmann R, Sträter N.