General Information
-
DRAMP ID
- DRAMP02819
-
Peptide Name
- Anoplin (Insects, arthropods, invertebrates, animals)
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Source
- Anoplius samariensis (Solitary wasp)
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Family
- Not found
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Gene
- Not found
-
Sequence
- GLLKRIKTLL
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Sequence Length
- 10
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UniProt Entry
- P0C005
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.29614317]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=26 μg/mL);
- Gram-negative bacterium: Escherichia coli ATCC 25922 (MIC=13 μg/mL);
- Fungi: Candida albicans ATCC 10231 (MIC=19.5 μg/mL).
- [Ref.25316570]Gram-positive bacteria : Staphylococcus aureus (MIC=64 μg/ml), bacillus subtilis (MIC=32 μg/ml);
- Gram-negative bacteria : Escherichia coli (MIC=64 μg/ml), Peudomonas eruginosa (MIC=32 μg/ml).
- [Ref.27862650]Gram-positive bacteria : Mycobacteria smegmatis mc2 155 (MIC=200 μg/ml), Bacillus subtilis DELTA (MIC=300 μg/ml);
- Gram-negative bacteria : Escherichia coli DH5a (MIC=200 μg/ml), Pseudomonas aeruginosa PAO (MIC=100 μg/ml), Zymomonas mobilis 10988 (MIC=40 μg/ml);
- Fungi : Candida parapsilosis (MIC=15 μg/ml)
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Hemolytic Activity
-
- [Ref.25316570] 1% hemolysis at 12.5 μg/ml , 2% hemolysis at 25 μg/ml , 1% hemolysis at 50 μg/ml , 1.5% hemolysis at 100 μg/ml , 1.5% hemolysis at 200 μg/ml against mice red blood cells.
- [Ref.27862650]3% hemolysis at 100μg/ml , 6% hemolysis at 200μg/ml , 10% hemolysis at 300μg/ml , 12% hemolysis at 500μg/ml against human red blood cells
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Alpha helix
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Structure Description
- The peptide adopts a random coil structure in water and tends to fold into an
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Helical Wheel Diagram
- 2MJQ-> 
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Predicted Structure
- There is no predicted structure for DRAMP02819.
Physicochemical Information
-
Formula
- C54H103N15O12
Absent Amino Acids
- ACDEFHMNPQSVWY
Common Amino Acids
- L
Mass
- 1154.5
PI
- 11.17
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 5
Net Charge
- +3
-
Boman Index
- -3.05
Hydrophobicity
- 0.63
Aliphatic Index
- 195
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP02819
Comments Information
[Ref.29614317]Function
- Antimicrobial activity against the Gram-positive, Gram-negative bacteria and fungi.##[Ref.25316570]Function
Literature Information
- ·Literature 1
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Title
- Effects of truncation of the peptide chain on the secondary structure and bioactivities of palmitoylated anoplin.
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Pubmed ID
- 29614317
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Reference
- Peptides. 2018 Jun;104:7-14.
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Author
- Salas RL, Garcia JKDL, Miranda ACR, Rivera WL, Nellas RB, Sabido PMG.
- ·Literature 2
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Title
- Design of novel analogues of short antimicrobial peptide anoplin with improved antimicrobial activity
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Pubmed ID
- 25316570#27862650
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Reference
- J Pept Sci. 2014 Dec;20(12):945-51. doi: 10.1002/psc.2705.
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Author
- Yang Wang, Jianbo Chen, Xin Zheng, Xiaoli Yang, Panpan Ma,Ying Cai,Bangzhi Zhangb,and Yuan Chen