General Information

    • DRAMP ID

    • DRAMP02819

    • Peptide Name

    • Anoplin (Insects, arthropods, invertebrates, animals)

    • Source

    • Anoplius samariensis (Solitary wasp)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLLKRIKTLL

    • Sequence Length

    • 10

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.29614317]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=26 μg/mL);
      • Gram-negative bacterium: Escherichia coli ATCC 25922 (MIC=13 μg/mL);
      • Fungi: Candida albicans ATCC 10231 (MIC=19.5 μg/mL).
      • [Ref.25316570]Gram-positive bacteria : Staphylococcus aureus (MIC=64 μg/ml), bacillus subtilis (MIC=32 μg/ml);
      • Gram-negative bacteria : Escherichia coli (MIC=64 μg/ml), Peudomonas eruginosa (MIC=32 μg/ml).
      • [Ref.27862650]Gram-positive bacteria : Mycobacteria smegmatis mc2 155 (MIC=200 μg/ml), Bacillus subtilis DELTA (MIC=300 μg/ml);
      • Gram-negative bacteria : Escherichia coli DH5a (MIC=200 μg/ml), Pseudomonas aeruginosa PAO (MIC=100 μg/ml), Zymomonas mobilis 10988 (MIC=40 μg/ml);
      • Fungi : Candida parapsilosis (MIC=15 μg/ml)

    • Hemolytic Activity

      • [Ref.25316570] 1% hemolysis at 12.5 μg/ml , 2% hemolysis at 25 μg/ml , 1% hemolysis at 50 μg/ml , 1.5% hemolysis at 100 μg/ml , 1.5% hemolysis at 200 μg/ml against mice red blood cells.
      • [Ref.27862650]3% hemolysis at 100μg/ml , 6% hemolysis at 200μg/ml , 10% hemolysis at 300μg/ml , 12% hemolysis at 500μg/ml against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • The peptide adopts a random coil structure in water and tends to fold into an

    • Helical Wheel Diagram

    • DRAMP02819 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP02819.

Physicochemical Information

    • Formula

    • C54H103N15O12

    • Absent Amino Acids

    • ACDEFHMNPQSVWY

    • Common Amino Acids

    • L

    • Mass

    • 1154.5

    • PI

    • 11.17

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +3

    • Boman Index

    • -3.05

    • Hydrophobicity

    • 0.63

    • Aliphatic Index

    • 195

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP02819

Comments Information

    • [Ref.29614317]Function

    • Antimicrobial activity against the Gram-positive, Gram-negative bacteria and fungi.##[Ref.25316570]Function

Literature Information

  • ·Literature 1

    • Title

    • Effects of truncation of the peptide chain on the secondary structure and bioactivities of palmitoylated anoplin.

    • Reference

    • Peptides. 2018 Jun;104:7-14.

    • Author

    • Salas RL, Garcia JKDL, Miranda ACR, Rivera WL, Nellas RB, Sabido PMG.
  • ·Literature 2

    • Title

    • Design of novel analogues of short antimicrobial peptide anoplin with improved antimicrobial activity

    • Reference

    • J Pept Sci. 2014 Dec;20(12):945-51. doi: 10.1002/psc.2705.

    • Author

    • Yang Wang, Jianbo Chen, Xin Zheng, Xiaoli Yang, Panpan Ma,Ying Cai,Bangzhi Zhangb,and Yuan Chen