General Information

    • DRAMP ID

    • DRAMP02912

    • Peptide Name

    • SMAP-29 (Cathelin-related peptide SC5; Myeloid antibacterial peptide MAP-29; mammals, animals)

    • Source

    • Ovis aries (Sheep)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • Not found

    • Sequence

    • RGLRRLGRKIAHGVKKYGPTVLRIIRIAG

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Antifungal

    • Target Organism

      • [Ref.8549789][No NaCl]: Escherichia coli DH5a (MIC=0.1 µM), Escherichia coli ML-35p (MIC=0.18 µM), Pseudomonas aeruginosa PAO1 (MIC=0.17 µM), Pseudomonas aeruginosa MR3007 (MIC=0.34 µM), Staphylococcus aureus ATCC (MRSA) 33591 (MIC=1.24 µM), Staphylococcus aureus 93918 (MIC=0.31 µM).
      • [100 mM NaCl]: Escherichia coli DH5a (MIC=0.07 µM), Escherichia coli ML-35p (MIC=0.12 µM), Pseudomonas aeruginosa PAO1 (MIC=0.08 µM), Pseudomonas aeruginosa MR3007 (MIC=0.18 µM), Staphylococcus aureus ATCC (MRSA) 33591 (MIC=0.65 µM), Staphylococcus aureus 93918 (MIC=0.43 µM).

    • Hemolytic Activity

      • [Ref.8549789]Not found

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Outer membrane protein I (OprI)

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02912 helical wheel diagram

    • PDB ID

    • 1FRY resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP02912.

Physicochemical Information

    • Formula

    • C146H260N52O32

    • Absent Amino Acids

    • CDEFMNQSW

    • Common Amino Acids

    • R

    • Mass

    • 3256

    • PI

    • 12.31

    • Basic Residues

    • 10

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +10

    • Boman Index

    • -62.7

    • Hydrophobicity

    • -0.21

    • Aliphatic Index

    • 121.03

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 53.21

    • Polar Residues

    • 7

DRAMP02912

DRAMP02912 chydropathy plot

Comments Information

    • Function

    • Broad spectrum bactericidal agent.

    • Biophysicochemical properties

    • Temperature dependence (Thermostable).

Literature Information

  • ·Literature 1

    • Title

    • Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide.

    • Reference

    • FEBS Lett. 1995 Dec 27;377(3):519-522.

    • Author

    • Mahoney MM, Lee AY, Brezinski-Caliguri DJ, Huttner KM.
  • ·Literature 2

    • Title

    • Impact of single-residue mutations on the structure and Function: of ovispirin/novispirin antimicrobial peptides.

    • Reference

    • Protein Eng. 2002 Mar;15(3):225-232.

    • Author

    • Sawai MV, Waring AJ, Kearney WR, McCray PB Jr, Forsyth WR, Lehrer RI, Tack BF.