General Information

    • DRAMP ID

    • DRAMP02935

    • Peptide Name

    • Polyphemusin-2 (Polyphemusin II; crabs, Arthropods, animals)

    • Source

    • Limulus polyphemus (Atlantic horseshoe crab)

    • Family

    • Belongs to the tachyplesin/polyphemusin family

    • Gene

    • Not found

    • Sequence

    • RRWCFRVCYKGFCYRKCR

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Antifungal

    • Target Organism

      • Yeast: Candida albicans M9.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys4 and Cys17,Cys8 and Cys13.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02935 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02935.

Physicochemical Information

    • Formula

    • C108H164N36O21S4

    • Absent Amino Acids

    • ADEHILMNPQST

    • Common Amino Acids

    • R

    • Mass

    • 2430.96

    • PI

    • 10.1

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +7

    • Boman Index

    • -67.59

    • Hydrophobicity

    • -0.8

    • Aliphatic Index

    • 16.11

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 8730

    • Absorbance 280nm

    • 513.53

    • Polar Residues

    • 7

DRAMP02935

DRAMP02935 chydropathy plot

Comments Information

    • Function

    • Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.

    • Tissue specificity

    • Hemocytes.

    • PTM

    • Contains two disulfide bonds 4-17; 8-13.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides, isolated from horseshoe crab hemocytes, tachyplesin II, and polyphemusins I and II: chemical structures and biological activity.

    • Reference

    • J Biochem. 1989 Oct;106(4):663-668.

    • Author

    • Miyata T, Tokunaga F, Yonega T, Yoshikawa K, Iwanaga S, Niwa M, Takao T, Shimonishi Y.
  • ·Literature 2

    • Title

    • Structure-activity relationships for the beta-hairpin cationic antimicrobial peptide polyphemusin I.

    • Reference

    • Biochim Biophys Acta. 2004 May 6;1698(2):239-250.

    • Author

    • Powers J.P, Rozek A, Hancock R.E.