General Information

    • DRAMP ID

    • DRAMP02961

    • Peptide Name

    • Antibacterial peptide PMAP-37 (Myeloid antibacterial peptide 37; pigs, mammals, animals)

    • Source

    • Sus scrofa (Pig)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • PMAP37

    • Sequence

    • GLLSRLRDFLSDRGRRLGEKIERIGQKIKDLSEFFQS

    • Sequence Length

    • 37

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Escherichia coli ML35 (MIC=2 µM), Escherichia coli ATCC 25922 (MIC=1 µM), Escherichia coli D21 (MIC=2 µM), Salmonella typhimurium ATCC 14028 (MIC=4 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=2 µM).
      • Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=4 µM), Bacillus megaterium local isolate (IC=4 µM), Staphylococcus aureus ATCC 25923 (MIC=32 µM), Staphylococcus aureus Cowan 1 (MIC=64 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02961 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02961.

Physicochemical Information

    • Formula

    • C192H320N60O56

    • Absent Amino Acids

    • ACHMNPTVWY

    • Common Amino Acids

    • LR

    • Mass

    • 4365.02

    • PI

    • 10.24

    • Basic Residues

    • 9

    • Acidic Residues

    • 6

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +3

    • Boman Index

    • -120.46

    • Hydrophobicity

    • -0.724

    • Aliphatic Index

    • 94.86

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 8

DRAMP02961

DRAMP02961 chydropathy plot

Comments Information

    • Function

    • Exerts antimicrobial activity against both Gram-positive and negative bacteria with minimal inhibitory concentrations ranging over 1-4 micro molar. Its activity appears to be mediated by its ability to damage bacterial membranes.

    • PTM

    • Contains two disulfide bonds (By similarity).

Literature Information

  • ·Literature 1

    • Title

    • PMAP-37, a novel antibacterial peptide from pig myeloid cells. cDNA cloning, chemical synthesis and activity.

    • Reference

    • Eur J Biochem. 1995 Mar 15;228(3):941-946.

    • Author

    • Tossi A, Scocchi M, Zanetti M, Storici P, Gennaro R.