General Information

    • DRAMP ID

    • DRAMP02970

    • Peptide Name

    • Protegrin-1 (Protegrin 1; PG-1; pigs, mammals, animals)

    • Source

    • Sus scrofa (Pig)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • NPG1

    • Sequence

    • RGGRLCYCRRRFCVCVGR

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Escherichia coli (MIC=64 µg/ml);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=64 µg/ml), Staphylococcus epidermidis (MIC=2 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.31399625] Cytotoxicity: 661W cells(IC50=353.4±70.5μM), HEK293T cells(IC50=2760±54.1μM), NIH-3T3 cells(IC50=719.2±59.5μM), SH-SY5Y cells(IC50=590.4±58.2μM), PMN cells(IC50=948.9±64.1μM), and 3D4/2 cells(IC50=391.1±71.3μM)

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys6 and Cys15,Cys8 and Cys13.

    • Stereochemistry

    • L

    • Structure

    • Beta strand

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02970 helical wheel diagram

    • PDB ID

    • 1PG1 resolved by NMR.
  • 1PG1-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C88H150N36O20S4

    • Absent Amino Acids

    • ADEHIKMNPQSTW

    • Common Amino Acids

    • R

    • Mass

    • 2160.63

    • PI

    • 10.66

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +6

    • Boman Index

    • -65.74

    • Hydrophobicity

    • -0.25

    • Aliphatic Index

    • 53.89

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1740

    • Absorbance 280nm

    • 102.35

    • Polar Residues

    • 8

DRAMP02970

DRAMP02970 chydropathy plot

Comments Information

    • The sequence of IB-367 is RGGLCYCRGRFCVCVGR and the peptide is active against bacteria and fungi. This peptide showed synergistic effect with LL-37 against P. aeruginosa and E. coli (Yan H & Hancock REW 2001 Antimicrob Agents Chemother 45

    • 1558-60). PG-1 derivatives were subjected to clinical trials.

Literature Information

  • ·Literature 1

    • Title

    • Synthesis of protegrin-related peptides and their antibacterial and anti-human immunodeficiency virus activity.

    • Reference

    • Chem Pharm Bull (Tokyo). 1995 May;43(5):853-858.

    • Author

    • Tamamura H, Murakami T, Horiuchi S, Sugihara K, Otaka A, Takada W, Ibuka T, Waki M, Yamamoto N, Fujii N.