General Information

    • DRAMP ID

    • DRAMP02971

    • Peptide Name

    • Protegrin-2 (Protegrin 2; PG-2; pigs, mammals, animals)

    • Source

    • Sus scrofa (Pig)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • NPG2

    • Sequence

    • RGGRLCYCRRRFCICV

    • Sequence Length

    • 16

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Antifungal, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Listeria moncyrogenes strain EGD (MIC>25 µg/ml);
      • Gram-negative bacterium: Escherichia coli ML-35 (MIC>25 µg/ml).
      • Yeast: Candida albicans strain 820 (MIC>25 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02971 helical wheel diagram

    • PDB ID

    • 2MUH resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP02971.

Physicochemical Information

    • Formula

    • C81H137N31O18S4

    • Absent Amino Acids

    • ADEHKMNPQSTW

    • Common Amino Acids

    • R

    • Mass

    • 1961.42

    • PI

    • 9.88

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +5

    • Boman Index

    • -50.88

    • Hydrophobicity

    • 0.044

    • Aliphatic Index

    • 66.88

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1740

    • Absorbance 280nm

    • 116

    • Polar Residues

    • 7

DRAMP02971

Comments Information

    • Function

    • Microbicidal activity. Active against E.coli, Listeria monocytogenes and C.albicans, in vitro.

    • PTM

    • Contains two disulfide bonds 6-15; 8-13 and Valine amide at V16.

Literature Information

  • ·Literature 1

    • Title

    • Protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins.

    • Reference

    • FEBS Lett. 1993 Jul 26;327(2):231-236.

    • Author

    • Kokryakov VN, Harwig SS, Panyutich EA, Shevchenko AA, Aleshina GM, Shamova OV, Korneva HA, Lehrer RI.
  • ·Literature 2

    • Title

    • A novel cDNA sequence encoding a pig leukocyte antimicrobial peptide with a cathelin-like pro-sequence.

    • Reference

    • Biochem Biophys Res Commun. 1993 Nov 15;196(3):1363-1368.

    • Author

    • Storici P, Zanetti M.