• • DRAMP ID

  • DRAMP03000

• • Peptide Name

  • Jellein-2 (Jelleine-II; chain of Major royal jelly protein 1; Insects, animals)

• • Source

  • Apis mellifera (Honeybee)

• • Family

  • Belongs to the major royal jelly protein family

• • Gene

  • MRJP1

• • Sequence

  • TPFKISIHL

• • Sequence Length

  • 9

• • UniProt Entry

  • O18330,Q548D6

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • Gram-positive bacteria: Staphylococcus aureus ATCC 6535 (MIC=15 µg/ml), S. saprophyticus (MIC=10 µg/ml), Bacillus subtilis CCT 2471 (MIC=30 µg/ml);
    • Gram-negative bacteria: Escherichia coli CCT 1371 (MIC=15 µg/ml), Enterobacter cloacae ACCT 23355 (MIC=15 µg/ml), Klebsiella pneumoniae ACCT 13883 (MIC=15 µg/ml), Pseudomonas aeruginosa ACCT 27853 (MIC=15 µg/ml).
    • Yeast: Candida albicans (MIC=2.5 µg/ml).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP03000.

DRAMP03000

• • Function

  • Jellein-2 has antibacterial and antifungal activity. Jellein-2 is probably processed to yield jellein-1 and jellein-4.

• • Tissue specificity

  • Found in the hypopharyngeal glands of the worker honeybee.

• • Developmental stage

  • Produced in the cephalic glands of both the nurse bee and the forager bee. This bee milk protein changes to alpha-glucosidase in accordance with the age-dependent role change of the worker bee.

• • Miscellaneous

  • Exhibits a growth factor-like action on primary-cultured rat hepatocytes by stimulating DNA synthesis and protecting cells from apoptosis induced by serum deprivation. Also activates mitogen-activated protein kinase, as well as protein kinase B, a key regulator of cell survival. Degraded proportionally to the period of storage, and is completely lost during storage at 40 degrees Celsius for 30 days. PTM

• ·Literature 1

• • Title

  • Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera).

• • Pubmed ID

  • 15203237

• • Reference

  • Peptides. 2004 Jun;25(6):919-928.

• • Author

  • Fontana R, Mendes MA, de Souza BM, Konno K, César LM, Malaspina O, Palma MS.

• ·Literature 2

• • Title

  • Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L.

• • Pubmed ID

  • 9395329

• • Reference

  • Eur J Biochem. 1997 Nov 1;249(3):797-802.

• • Author

  • Ohashi K, Natori S, Kubo T.

• ·Literature 3

• • Title

  • Royalactin induces queen differentiation in honeybees.

• • Pubmed ID

  • 21516106

• • Reference

  • Nature. 2011 May 26;473(7348):478-483.

• • Author

  • Kamakura M.