General Information

    • DRAMP ID

    • DRAMP03003

    • Peptide Name

    • Melectin (MEP; Insects, animals)

    • Source

    • Melecta albifrons (Cuckoo bee) (Melecta punctata)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GFLSILKKVLPKVMAHMK

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.18942691]Gram-positive bacteria: Bacillus subtilis (MIC=0.8 µM), Staphylococcus aureus (MIC=6.8 µM);
      • Gram-negative bacteria: Escherichia coli (MIC=2.0 µM), Pseudomonas aeruginosa (MIC=18.5 µM).

    • Hemolytic Activity

      • [Ref.18942691]LC50>100 μM against rat red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03003 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C96H166N24O20S2

    • Absent Amino Acids

    • CDENQRTWY

    • Common Amino Acids

    • K

    • Mass

    • 2040.64

    • PI

    • 10.48

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +5

    • Boman Index

    • 7.93

    • Hydrophobicity

    • 0.617

    • Aliphatic Index

    • 124.44

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP03003

DRAMP03003 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity against Gram-positive bacteria as well as Gram-negative bacteria. Stimulates degranulation from rat peritoneal mast cells with an EC50 of 19.4 µM. Has weak hemolytic activity towards rat erythrocytes.

    • Tissue specificity

    • Expressed by the venom gland.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Melectin: a novel antimicrobial peptide from the venom of the cleptoparasitic bee Melecta albifrons.

    • Reference

    • Chembiochem. 2008 Nov 24;9(17):2815-2821.

    • Author

    • Cerovský V, Hovorka O, Cvacka J, Voburka Z, Bednárová L, Borovicková L, Slaninová J, Fucík V.