General Information

    • DRAMP ID

    • DRAMP03024

    • Peptide Name

    • Mastoparan B (MP-B; insects, arthropods, invertebrates, animals)

    • Source

    • Vespa basalis (Hornet)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • LKLKSIVSWAKKVL

    • Sequence Length

    • 14

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anti Mammalian cells, Anti-cancer

    • Target Organism

      • [Ref.10667861] Gram-positive bacteria: Staphylococcus epidermidis ATTC 12228 (MIC=25 µM), Bacillus subtilis PCI 219 (MIC=25 µM), Enterococcus faecium EFMY-28 (MIC=12.5 µM);
      • Gram-negative bacterium: Escherichia coli NIHJ JC-2 (MIC=50 µM)

    • Hemolytic Activity

      • [Ref.10667861] 68.2% hemolysis at the peptide concentration of 100 µM is , 27.9% Hemolysis at 50 µM against human red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Tryptophan residue in MP

    • Helical Wheel Diagram

    • DRAMP03024 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C78H137N19O17

    • Absent Amino Acids

    • CDEFGHMNPQRTY

    • Common Amino Acids

    • K

    • Mass

    • 1613.06

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • 2.9

    • Hydrophobicity

    • 0.571

    • Aliphatic Index

    • 160

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 423.08

    • Polar Residues

    • 2

DRAMP03024

Comments Information

    • Function

    • Mast cell degranulating peptide. Activates G proteins that couple to phospholipase C. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Relationship between the tertiary structures of mastoparan B and its analogs and their lytic activities studied by NMR spectroscopy.

    • Reference

    • J Pept Res. 2000 Jan;55(1):51-62.

    • Author

    • Yu K, Kang S, Park N, Shin J, Kim Y.
  • ·Literature 2

    • Title

    • Interaction of mastoparan-B from venom of a hornet in Taiwan with phospholipid bilayers and its antimicrobial activity

    • Reference

    • Biopolymers. 1995 Dec;36(6):793-801

    • Author

    • Park NG, Yamato Y, Lee S, Sugihara G1995