General Information

    • DRAMP ID

    • DRAMP03037

    • Peptide Name

    • Eumenitin (Er-12; Insects, animals)

    • Source

    • Eumenes rubronotatus (Solitary wasp)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • LNLKGIFKKVASLLT

    • Sequence Length

    • 15

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.16762455]Gram-positive bacteria: Staphylococcus aureus ATCC 6538 (MIC>60 µM), Staphylococcus aureus ATCC 25923 (MIC=6 µM), Staphylococcus saprophytius (CS) (MIC=6 µM), Staphylococcus epidermius (CS) (MIC>60 µM), Bacillus subtilis CCT 2471 (MIC=60 µM), Bacillus thuringiensis (WT) (MIC>60 µM);
      • Gram-negative bacteria: Escherichia coli CCT 1371 (MIC=6 µM), Escherichia coli ATCC 25922 (MIC=6 µM), Escherichia cloacae ATCC 23355 (MIC>60 µM), Proteus mirabilis (CS) (MIC>60 µM), Pseudomonas aeruginosa ATCC 15442 (MIC=30 µM).

    • Hemolytic Activity

      • [Ref.16762455]Non-hemolytic activity against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane (Note: Assumes an amphipathic alpha-helical conformation in a lipid environmen

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03037 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C78H137N19O19

    • Absent Amino Acids

    • CDEHMPQRWY

    • Common Amino Acids

    • L

    • Mass

    • 1645.06

    • PI

    • 10.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +3

    • Boman Index

    • 5.11

    • Hydrophobicity

    • 0.76

    • Aliphatic Index

    • 156

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP03037

Comments Information

    • Function

    • Cationic linear alpha-helical antimicrobial peptide that binds preferentially to charged lipid membranes as compared with zwitterionic ones. It exhibits inhibitory activity against both Gram-positive and Gram-negative bacteria, and moderately stimulates degranulation from the rat peritoneal mast cells. Does not show hemolytic activity. May play a role in preventing infection by microorganisms during prey consumption by their larvae.

    • Tissue specificity

    • Expressed by the venom gland.

Literature Information

  • ·Literature 1

    • Title

    • Eumenitin, a novel antimicrobial peptide from the venom of the solitary eumenine wasp Eumenes rubronotatus.

    • Reference

    • Peptides. 2006 Nov;27(11):2624-2631.

    • Author

    • Konno K, Hisada M, Naoki H, Itagaki Y, Fontana R, Rangel M, Oliveira JS, Cabrera MP, Neto JR, Hide I, Nakata Y, Yasuhara T, Nakajima T.
  • ·Literature 2

    • Title

    • Effects of the cationic antimicrobial peptide eumenitin from the venom of solitary wasp Eumenes rubronotatus in planar lipid bilayers: surface charge and pore formation activity.

    • Reference

    • Toxicon. 2008 Apr;51(5):736-745.

    • Author

    • Arcisio-Miranda M, dos Santos Cabrera MP, Konno K, Rangel M, Procopio J.