General Information

    • DRAMP ID

    • DRAMP03046

    • Peptide Name

    • Orancis-protonectin (chain of Venom peptide 2-long; Venom peptide 2, OdVP2; Insects, animals)

    • Source

    • Orancistrocerus drewseni (Solitary wasp)

    • Family

    • Belongs to the MCD family (Protonectin subfamily)

    • Gene

    • VP2

    • Sequence

    • ILGIITSLLKSL

    • Sequence Length

    • 12

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

    • Target Organism

      • [Ref.19857508]Gram-positive bacteria: Staphylococcus aureus (MIC=25 μg/ml);
      • Fungi: Candida albicans (MIC<100 µg/ml), Botrytis cinerea (MIC=0.5 µg/ml).

    • Hemolytic Activity

      • [Ref.18695936]84% hemolytic activity at 50 μM against sheep blood cells.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03046 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03046.

Physicochemical Information

    • Formula

    • C60H111N13O16

    • Absent Amino Acids

    • ACDEFHMNPQRVWY

    • Common Amino Acids

    • L

    • Mass

    • 1270.62

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +1

    • Boman Index

    • 20.46

    • Hydrophobicity

    • 1.842

    • Aliphatic Index

    • 227.5

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP03046

Comments Information

    • Function

    • Has potent hemolytic activity against ovine erythrocytes at micromolar concentrations. Also exhibits strong antimicrobial activity against fungi. Venom peptide 2-long has substantially lower antimicrobial activity against C. albicans and bacteria than Orancis-protonectin but has equivalent activity against Botrytis cinerea.

Literature Information

  • ·Literature 1

    • Title

    • Isolation and molecular cloning of venom peptides from Orancistrocerus drewseni (Hymenoptera: Eumenidae).

    • Reference

    • Toxicon. 2010 Apr 1;55(4):711-718.

    • Author

    • Baek JH, Lee SH.
  • ·Literature 2

    • Title

    • Novel mastoparan and protonectin analogs isolated from a solitary wasp, Orancistrocerus drewseni drewseni.

    • Reference

    • Amino Acids. 2009 Jul;37(2):389-394.

    • Author

    • Murata K, Shinada T, Ohfune Y, Hisada M, Yasuda A, Naoki H, Nakajima T.